《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (12): 1101-1102.

• 专题　脑瘫康复 •    下一篇

针刺对宫内感染致脑损伤仔鼠脑组织碱性成纤维细胞生长因子表达的影响

李晓捷; 迟瑛娇; 庞伟   

  1. 佳木斯大学康复医学院儿童神经康复实验室,黑龙江佳木斯市 154002
  • 收稿日期:2007-07-18 出版日期:2007-12-01 发布日期:2007-12-01

Effect of Acupuncture on Expression of Basic Fibroblast Growth Factor in Brain of Neonatal Rats Injured by Intrauterine Infection

LI Xiao-jie, CHI Ying-jiao, PANG Wei   

  1. College of Rehabilitation Medicine, Jiamusi University, Jiamusi 154002, Heilongjiang,China
  • Received:2007-07-18 Published:2007-12-01 Online:2007-12-01

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被引次数

75

摘要： 目的观察针刺对宫内感染致脑损伤仔鼠脑组织碱性成纤维细胞生长因子(bFGF)表达的影响。方法对孕17、18d的Wistar大鼠腹腔注射脂多糖建立动物模型,以腹腔注射生理盐水为对照组。正常分娩后,仔鼠随机分为针刺组与模型组,针刺组7～21日龄时给予针刺干预。3组分别于生后21d取幼鼠脑组织应用免疫组化方法检测脑白质bFGF的表达。结果针刺组bFGF免疫阳性细胞数量多,染色深;对照组bFGF免疫阳性细胞数量少,染色浅;模型组介于两者之间。结论bFGF表达增加可能是针刺对宫内感染致脑损伤仔鼠的治疗机制之一。

关键词: 宫内感染, 脑白质, 针刺, 碱性成纤维细胞生长因子(bFGF)

Abstract: Objective To observe the effect of acupuncture on expression of basic fibroblast growth factor (bFGF) in brain of neonatal rats injured by intrauterine infection. Methods 43 pregnant Wistar rats were divided into two groups: lipopolysaccharide (LPS, n=35) and normal saline (n=8, as control), which were consecutively injected intraperitoneally with LPS (450 μg/kg) or saline on the 17th and 18th day of gestation. LPS group was randomly divided into the acupuncture group and model group. Acupuncture group was given acupuncture 7～21 d after being born. bFGF expression was assayed with immunohistochemistry. Results The number of bFGF positive neurons in the cerebral white matter was large in the acupuncture group, medium in the model group and little in the control group. Conclusion Acupuncture may be used to treat brain injury caused by intrauterine infection at the early stage, which may result from its up-regulating the expression of bFGF in the cerebral white matter.

Key words: intrauterine infection, white matter, acupuncture, basic fibroblast growth factor (bFGF)