《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (09): 842-844.

• 基础研究 • 上一篇    下一篇

钾离子通道相互作用蛋白1与γ-氨基丁酸能神经元在戊四唑致癫痫大鼠海马部位的改变

罗爱华1a; 廖卫平1b; 苏涛1b; 孙卫文1b; 张灼华2   

  1. 1.广州医学院第二附属医院a.康复科;b.神经内科,广州医学院神经科学研究所，广东广州市 510260;2.Center for Neuroscience and Aging,the Burnham Institute University of California; CA92037; San Diego,USA
  • 收稿日期:2007-04-26 出版日期:2007-09-01 发布日期:2007-09-01

Change of Kv Channel Interacting Protein 1 and Gamma-Aminobutyric Acid-ergic Neurons in Hippocampus of Pentylenetetrazole Induced Epilepsy Rats

LUO Ai-hua, LIAO Wei-ping, SU Tao, et al   

  1. Department of Rehabilitation, the2nd Affiliated Hospital of Guangzhou Medical College, Guangzhou510260, Guangdong, China
  • Received:2007-04-26 Published:2007-09-01 Online:2007-09-01

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摘要： 目的研究钾离子通道相互作用蛋白1(KChIP1)在癫痫发生过程中的变化及其与γ-氨基丁酸(GABA)能神经元的关系。方法成年大鼠制作急性戊四唑癫痫模型,免疫组织化学法结合激光扫描共聚焦显微镜技术观察大鼠脑中KChIP1免疫阳性神经元与GABA能阳性神经元在海马部位的表达及改变。结果急性戊四唑癫痫大鼠海马部KChIP1阳性神经元数较对照组明显增加(P<0.05),GABA能阳性神经元、KChIP1/GABA双标阳性神经元数与对照组无显著性差异(P>0.05);KChIP1和GABA能阳性神经元在海马部位的共存率约为63.9%。结论KChIP1在癫痫发生中可能起重要作用;KChIP1与GABA能神经元虽有密切的共存关系,但在功能上并不完全一致。

关键词: 癫痫, 钾离子通道相互作用蛋白1(KChIP1), γ-氨基丁酸能神经元, 戊四唑, 海马, 大鼠

Abstract: Objective To explore the role of Kv channel interacting protein 1(KChIP1)in the process of epileptic seizure and the relationship between KChIP1 and gamma-aminobutyric acid-(GABA)ergic neurons.Methods Normal female Sprague-Dawley rats were treated with pentylenetetrazole to make acute pentylenetetrazole models of epilepsy.Laser Scanning Confocal Microscope(LSCM)combined with double-labeled immunohistochemical technique was applied to observe the expression of the KChIP1 and the GABAergic neurons in the hippocampus of rats.Results The number of KChIP1-postive neurons in the hippocampus was significantly increased in the acute pentylenetetrazole model rats(P<0.05).There was no significant difference in the number of double-labled neurons(P>0.05),nor of the GABA-postive neurons between the model rats and the controls.The ratio of double-labeled neurons/total positive neurons was 63.9% in the hippocampus.Conclusion The KChIP1 might be involved in epileptogenesis of pentylenetetrazole induced seizure.The KChIP1 was associated with GABAergic neurons,whereas it may be functionally different from GABA.

Key words: epilepsy, Kv channel interacting protein1(KChIP1), GABAergic neuron, pentylenetetrazole, hippocampus, rats