《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (08): 733-735.

• 基础研究 • 上一篇    下一篇

类先天性马蹄足动物模型胚胎病理初步研究及病因学意义

贾艳丽1a; 杜世新1b; 刘戈飞2   

  1. 1.汕头大学医学院第一附属医院a.矫形外科;b.骨科,广东汕头市 515041;2.汕头大学医学院细胞生物实验室,广东汕头市 515031
  • 收稿日期:2007-05-15 出版日期:2007-08-01 发布日期:2007-08-01

Primary Research and Etiological Implication of Congenital Clubfoot-like Model at Pathological and Molecule Level

JIA Yan-li, DU Shi-xin, LIU Ge-fei   

  1. The Department of Orthopaedics, the First Clinical College of Shantou University, Shantou 515041, Guangdong, China
  • Received:2007-05-15 Published:2007-08-01 Online:2007-08-01

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摘要： 目的分析在肢体发育不同时段综合多基因相互作用条件下最终导致先天性马蹄内翻足(CCF)发生的原因。方法77只大白鼠从怀孕第10天起,将维甲酸石蜡油混悬液经胃管单次注入,建立CCF动物模型;应用光镜、电镜及分子生物学方法检测胎鼠后肢芽(后肢)、胸腰段脊髓。结果动物模型的CCF发病率为61.8%,距骨发育停滞,距骨、跟骨间重叠不良和跟骨内翻,后肢芽及脊髓前角细胞均有凋亡现象。结论CCF在胚胎发育期即有畸形,且程度随生长发育而逐步加重,后肢芽及脊髓前角细胞在胚胎早期即出现凋亡现象。

关键词: 足畸形, 先天性, 维甲酸, 凋亡

Abstract: Objective To explore the possible correlations under the interaction of multi-genes action at different stages and analyze the primary histophotomacrographic changes of hind buds tissue in congenital clubfoot and the pathodynamic developmental procedure.MethodsSeventy-seven female Wistar rats were administered with retinoic acid on the 10th day after pregnancy. And the hindlimb, buds and spinal cord were detected through transmission electron microscopic and molecular biological experiments.ResultsThere was clubfoot-like deformity in 61.8% of the experimental animals. Persistence of the embryonic position of the talus and tibia in fetuses was observed. Poor overlapping between talus and calcaneus was seen. Cell apoptosis at the anterior corner of spinal cord and hind buds were seen.ConclusionCongenital clubfoot deformities appear at early stage and exaggerate along with developmental procedure.

Key words: foot deformities, congenital, retinoic acid, apoptosis