《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (08): 730-732.

• 基础研究 • 上一篇    下一篇

电针对DPN大鼠坐骨神经神经生长因子调节作用的实验研究

东红升1; 张秋娟2; 杨强2; 郑敏2   

  1. 1.上海市针灸经络研究所,上海市 200030;2.上海中医药大学附属岳阳中西医结合医院,上海市 200473
  • 收稿日期:2007-05-15 出版日期:2007-08-01 发布日期:2007-08-01

Experimental Study of Nerve Growth Factor of Sciatic Nerve of Rats with Diabetes Peripheral Neuropathy Accommodated by Electro-acupuncture

DONG Hong-sheng, ZHANG Qiu-juan, YANG Qiang, et al   

  1. Shanghai Institute of Acupuncture-Moxibustion Meridians, Shanghai 200030, China
  • Received:2007-05-15 Published:2007-08-01 Online:2007-08-01

PDF (PC)

被引次数

89

摘要： 目的探讨电针治疗糖尿病周围神经病变(DPN)调节作用的机制。方法采用链脲佐菌素(STZ)制备DPN大鼠模型,将大鼠随机分为正常组、模型组、西药组和电针组;电针组取大鼠足三里、肾俞穴进行电针治疗,通过免疫组化和荧光定量PCR技术检测坐骨神经神经生长因子(NGF)蛋白和 mRNA的表达。结果模型组、西药组和电针组大鼠NGF蛋白和 mRNA的表达明显低于正常组(P<0.05～0.01);电针组大鼠治疗后坐骨神经NGF蛋白和 mRNA表达上调,明显高于模型组(P<0.01)。结论电针足三里、肾俞穴能够使DPN大鼠坐骨神经NGF蛋白和 mRNA表达上调,促进坐骨神经修复。

关键词: 坐骨神经, 神经营养因子, 糖尿病周围神经病变, 电针, 大鼠

Abstract: Objective To explore the mechanism of the nerve growth factor (NGF) accommodated by electro-acupuncture in rats with diabetes peripheral neuropathy (DPN).MethodsThe rat DPN model was established with streptozotocin (STZ). The rats were randomly divided into the normal group, model group, western medicine group and electro-acupuncture group. The animals of the electrol-acupuncture group were treated with electrol-acupuncture on "Zusanli" and "Shenshu" after DPN model established. The nervous tissues of all animals were examined with the techniques of immunity and fluorescent quantitation PCR to detect the albumen and mRNA expression of NGF.ResultsThe albumen and mRNA expression of NGF of the animals in the model group, western medicine group and electro-acupuncture group were significantly lower than that in the normal group ( P<0.05～0.01), and the albumen and mRNA expression of NGF was up-regulation in the electro-acupuncture group after electro-acupuncture treatment and was significantly higher than that in the model group ( P<0.01).ConclusionElectro-acupuncture on "Zusanli" and "Shenshu" can up-regulate the albumen and mRNA expression of NGF, and improve reparation of the sciatic nerve in DPN animals.

Key words: sciatic nerve, nerve growth factor (NGF), diabetes peripheral neuropathy (DPN), electro-acupuncture, rat