《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (08): 701-703.

• 专题　神经损伤康复 •    下一篇

银杏内酯B对神经干细胞分化的影响及其机制研究

王永红1,2; 罗雪2; 石永江1; 刘宏亮1; 姚忠祥2   

  1. 1.第三军医大学西南医院康复理疗科,重庆市 400038;2.第三军医大学组织胚胎学教研室,重庆市 400038
  • 收稿日期:2006-11-15 出版日期:2007-08-01 发布日期:2007-08-01

Mechanisms and Effect of Ginkgolide B on the Differentiation of Neuron Stem Cells

WANG Yong-hong, LUO Xue, SHI Yong-jiang, et al   

  1. The Department of Physical Medicine and Rehabilitation, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China
  • Received:2006-11-15 Published:2007-08-01 Online:2007-08-01

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被引次数

38

摘要： 目的观察银杏内酯B对体外培养的神经干细胞(NSCs)分化的影响。方法用含不同浓度银杏内酯B(20mg/L、40mg/L、60mg/L)的分化培养基培养NSCs3d和7d,测量细胞突起长度和胞体面积,用免疫荧光检测神经丝蛋白-200(NF-200)、胶质纤维酸性蛋白(GFAP)及少突胶质细胞特异性蛋白(CC-1)、细胞因子信号抑制因子2(SOCS2)、DNA结合抑制因子2(Id2)的表达。结果银杏内酯B组细胞突起增长,胞体增大;NF阳性神经元样细胞百分率增加,GFAP阳性星形胶质样细胞百分率随银杏内酯B浓度增加而增加;SOCS2阳性细胞百分率(35.46%)较对照组(22.17%)明显增加(P<0.01),银杏内酯B组Id2阳性细胞百分率(51.52%)较对照组(66.24%)明显减少(P<0.01)。结论银杏内酯B可促进分化细胞成熟,促进NSCs向神经元分化;星形胶质细胞百分率的增高与银杏内酯B呈剂量依赖关系。

关键词: 银杏内酯B, 神经干细胞, 分化, 细胞因子信号抑制因子2, DNA结合抑制因子2

Abstract: Objective To observe the effect of Ginkgolide B of various consistency on the differentiation of neuron stem cells (NSCs).MethodsNSCs were cultured in differentiation medium containing Ginkgolide B of various consistency for 3 and 7 days, the neurites length and cell body area were measured by inverted phase-contrast micrograph, then neurofilament-200 (NF-200), glial fibrillary acidic protein (GFAP), adenomatus polyposis coli (CC-1) expression were detected and counted by fluorescence microscope. The suppressor of cytokine signaling-2 (SOCS2), inhibitor of DNA binding-2 (Id2) were alsoimmunostained. The percentage of positive cells were counted respectively.ResultsThe neurites length and cell body area in Ginkgolide B groups were obviously larger than that in the control group. The percentage of NF, GFAP positive cells in Ginkgolide B groups increased with dosage increasing of Ginkgolide B. Compared with the normal control group, the percentage of SOCS2 positive cells increased significantly ( P<0.01) and the percentage of Id2 positive cells decreased significantly ( P<0.01) in Ginkgolide B groups.ConclusionGinkgolide B can promote NSCs to differentiate into neuron and astrocyte, the percentage of astrocyte is increased with a dosage-dependent relationship with Ginkgolide B.

Key words: Ginkgolide B, neuron stem cells, differentiation, suppressor of cytokine signaling-2 (SOCS2), inhibitor of DNA binding-2 (Id2)