《中国康复理论与实践》

《中国康复理论与实践》 ›› 2002, Vol. 8 ›› Issue (09): 520-522.

• 专题研究 • 上一篇    下一篇

降纤酶的不同给药途径和时间对大鼠局灶性脑缺血的影响

武小玲1; 尹岭2; 杜卫3; 王鲁宁2; 朱克2   

  1. 1.中国人民解放军第251医院 河北张家口市 075000;2.中国人民解放军总医院 北京市 100853;3.中国人民解放军第210医院 大连市 116021
  • 收稿日期:2002-05-23 出版日期:2002-09-25 发布日期:2002-09-25

Experimental study of the therapeutic effectiveness of defibrase on treating acute cerebral ischemia and the effectiveness in different administrated ways

WU Xiao-ling, YIN Lin,DU Wei,et al   

  1. Center of Neuroinformatics, General Hospital of PLA, Beijing 100853,China
  • Received:2002-05-23 Published:2002-09-25 Online:2002-09-25

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摘要： 目的探讨不同的给药途径下降纤酶去纤作用及其对局灶性脑缺血的影响,并分析静脉用药的治疗时间窗问题。方法采用雄性Wistar大鼠共178只,其中154只用线栓法制备局灶性脑缺血模型,被随机分为假手术组、降纤酶治疗组及生理盐水对照组,根据给药途径的不同每组又分为静脉给药和腹腔给药两个亚组。静脉给药组根据给药时间的不同又分为0.5、3、6、9、12h5个组。给予神经功能评分,脑标本用TTC染色以测量梗死灶体积,HE染色以观察病理改变。另24只大鼠分静脉及腹腔两种给药途径注射降纤酶,并在注药后0.5、3、6、12、2 4h采血,测定纤维蛋白原浓度。对比分析降纤酶不同给药途径及不同纤维蛋白原水平对疗效的影响及治疗时间窗的问题。结果静脉给药组的纤维蛋白原下降最快,3h下降至最低值;腹腔给药组的纤维蛋白原平缓下降,6h下降至最低。缺血后0.5、3h经静脉给药治疗组大鼠的神经功能评分改善、脑梗死体积最小,与降纤酶腹腔给药组、生理盐水对照组相比均有显著差异(P<0.05);降纤酶腹腔给药组与生理盐水对照组相比仅神经功能评分改善,梗死灶体积无显著差异(P>0.05)。缺血6、9、12h静脉给药组大鼠的神经功能评分、脑梗死体积与对照组比较均无明显差异(P>0.05)。结论 缺血早期静脉应用降纤酶可有效缩小大鼠的梗死灶体积。

关键词: 局灶脑缺血, 降纤酶, 梗死灶体积

Abstract: ObjectiveTo investigate the intensity of degrading plasma fibrinogen(FIB) and the therapeutic effectiveness of defibrase on treating cerebral ischemia by different administrated ways. MethodsIntraluminal suture method was used to develop reversible middle cerebral artery occlusion (MACO). 154 healthy male Wistar rats were randomized into 2 groups. The rats in intravenous treatment group were injected defibrase intravenously at 0.5,3,6,9,12hours after MACO,while the rats in coeliac treatment group were injected defibrase by abdominocentesis. Meanwhile the control group received normal saline. All rats were killed at 24 hours after MCAO. The thrombus in middle cerebral artery (MCA) and cerebral infarction were examined microscopically in HE stained sections. Infarction volume was measured by using 2,3,5-triphenyltetrazolium chloride staining. 24 rats were selected randomly and injected defibrase by intravenous injection and abdominocentesis. Plasma FIB was measured before and after injection 1,3,6,12,24h by intravenous haemospasia. ResultsPlasma FIB was significantly reduced in intravenous treatment group, and it was lowerest in 3h after intravenous treatment.Clinical Neurological Deficits Scale and infarction volume was significantly reduced in intravenous treatment group than saline control group and coeliac treatment group.There was improvement in Clinical Neurological Deficits Scale in coeliac treatment group compared with that of saline control group, but there was no statistically significant differences at infarction volume.Clinical Neurological Deficits Scale and infarction volume was statistically significant differences in intravenous treatment group at 0.5,3 hours after MCAO. There were no statistically significant differences in intravenous treatment group at 6, 9,12 hours after MCAO.Conclusions Defibrase can reduce the infarction volume in cerebral ischemia early stage.

Key words: focal cerebral ischemia, defibrase, infarction volume