《中国康复理论与实践》

《中国康复理论与实践》 ›› 2002, Vol. 8 ›› Issue (09): 516-519.

• 专题研究 • 上一篇    下一篇

中药参乌胶囊对老年大鼠学习记忆及海马组织学的影响

张兰; 李林; 李斌; 丰艳; 叶翠飞; 张丽; 陆华宝   

  1. 首都医科大学宣武医院药理研究室,北京脑老化重点实验室 北京市 100053
  • 收稿日期:2002-05-04 出版日期:2002-09-25 发布日期:2002-09-25

Effects of Sheng-wu Capsule on learning-memory ability and hippocampal histology in aged rats

Zhang Lan,Li Lin,Li Bin, et al   

  1. Department of Pharmacology, Beijing Key Laboratory for Brain Aging,Xuan-wu Hospital of Capital University of Medical Sciences,100053 Beijing,China
  • Received:2002-05-04 Published:2002-09-25 Online:2002-09-25

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摘要： 目的探讨中药复方参乌胶囊改善学习记忆功能的作用机理。方法雌性Wistar大鼠,老年为22月龄,青年为5月龄。参乌胶囊高剂量1.8g/kg,低剂量0.9g/kg,每日灌胃给药一次,连续灌胃2个月。通道式水迷宫检测动物学习记忆功能,HE染色显示神经元,ABC法行星形胶质细胞、神经生长因子(NGF)及其受体TrkA免疫组化染色。VISILOG 5.0图像分析系统对组织切片进行分析。记数海马CA1区总阳性细胞数量、阳性细胞总面积(像素单位)及阳性细胞灰度积分值(灰度单位)。结果老年大鼠水迷宫游出时间延长、错误次数增多(P<0.001);海马CA1区神经元数量减少(P<0.05),星形胶质细胞增生(P<0.01);NGF及其受体TrkA阳性细胞数、阳性细胞总面积和阳性细胞灰度积分值较青年对照减少(P<0.05)。参乌胶囊使老年大鼠水迷宫游出时间缩短、错误次数减少(P<0.01);海马CA1区神经元数量增加,星形胶质细胞增生减少(均P<0.01);NGF及其受体TrkA阳性细胞数、阳性细胞总面积和阳性细胞灰度积分值明显增多(P<0.01—0.05);参乌胶囊高剂量组基本恢复青年对照组水平。结论参乌胶囊能够明显促进老年大鼠海马区NGF及受体TrkA表达,增加神经元数量、减少胶质细胞增生,对大鼠学习记忆力有促进作用。

关键词: 中药, 学习记忆, 老年, 大鼠, 海马, 神经生长因子, TrkA受体

Abstract: ObjectiveTo investigate the effects of Chinese compound Sheng-wu Capsule on learning-memory ability and histology including the neurons, astrocytes and expression of nerve growth factor(NGF) and its receptor (TrkA) in hippocampus CA1 of aged rats. MethodsSheng-wu Capsule consists of 6 kinds of Chinese medicinal herbs such as polygonum multiflorum,ginseng, and Rhizoma Acori Tatarinowii.The water-soluble component of polygonum multiflorum,2,3,5,4'- tetrahydroxystibane-2-O-β-D-glucoside was the quality standard of Sheng-wu Capsule.Aged Wistar rats(22 months old) were orally administrated with Sheng-wu Capsule(1.8g/kg and 0.9g/kg) for 2 months. Positive control drug was Piracetam(0.56g/kg). The learning and memory ability was tested by passageway water maze; the number and morphology of neurons was detected by HE staining; the proliferation of astrocytes was detected by immuno-histochemistry for glial fibrillary acidic protein(GFAP); the expression of neurotrophic substance was detected by immuno-histochemistry for NGF and TrkA receptor by ABC method. Image analysis to determine average cell number, size(area) and staining density in hippocampal CA1 region was achieved by VISILOG 5.0 image pattern analyser.ResultsBoth NGF and TrkA-immunoreactive neurons in aged rats significantly decreased as well as cell number, size and staining density in CA1 region of hippocampus(P<0.01). Aged rats showed damage of neurons and increase of GFAP positive cells in the same area,model rats indicated learning-memory deficit too. In Sheng-wu Capsule administrated groups,the significant little decrease in cell number, size and staining density of NGF and TrkA-immunoreactive neurons in CA1 region of hippocampus was observed(P<0.01-0.05). The loss of Neurons and proliferation of astrocytes were inhibited and learning-memory ability was also improved in Sheng-wu groups.Conclusions Chinese compound Sheng-wu Capsule significantly promotes the expression of NGF and TrkA in aged rats and it relieves the damage of hippocampus.This may be the mechanism by which Sheng-wu Capsule improves the learning and memory ability. Enhancing endogenous neurotrophic substance plays an important role in the treatment of Alzheimer's disease.

Key words: Chinese herbs, learning-memory, aged, rats, hippocampus, nerve growth factor, TrkA receptor