《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (06): 526-527.

• 基础研究 • 上一篇    下一篇

血管组织工程实验研究

尹太1a; 徐迎新1b; 吴仕和1b; 王金晶1b; 鄂玲玲1b; 李荣1b; 李瑞新2; 张西正2   

  1. 1.解放军总医院a.血管外科;b.普通外科研究所,北京市 100853;2.军事医学科学院卫生装备研究所,天津市 300161
  • 收稿日期:2007-04-23 出版日期:2007-06-01 发布日期:2007-06-01

Experimental Study of Vascular Tissue Engineering

YIN Tai, XUYing-xin, WU Shi-he, et al   

  1. The Department of Blood Vascular Surgery, the General Hospital of PLA, Beijing 100853, China
  • Received:2007-04-23 Published:2007-06-01 Online:2007-06-01

摘要: 目的获得血管组织工程的实验依据。方法获取大鼠血管内皮(VEC)和平滑肌细胞(VSMCs),进行培养扩增,然后接种到血管组织工程支架上;将VSMCs-血管支架移植到大鼠体内,观察移植后大鼠活体和组织病理反应。结果培养的细胞符合血管种子细胞的形态特征;VSMCs接种到血管组织工程支架上获得成功;VSMCs-血管支架移植到大鼠体内10d后,支架被纤维组织包裹,支架内见VSMCs生长,支架中央管腔存在,但与正常血管结构有很大差别,而且不具备血管功能。结论利用血管组织工程支架构建的大鼠VSMCs-血管支架移植到大鼠皮下是可行的;人工构建血管具有类似血管雏形,但尚不具备血管的正常结构。

关键词: 组织工程, 血管, 壳聚糖, 血管移植, 支架

Abstract: Objective To obtain the experimental data of vascular tissue engineering.MethodsThe vascular endothelial cells (VEC) and vascular smooth muscle cells (VSMCs) were acquired and cultured, and then seeded on vascular tissue engineering materials. The porous gelatin-chitosan scaffold with VSMCs was subcutaneously implanted, followed by the observation of the cell growth ten days later.ResultsThe two kinds of cells were successfully cultured and their morpholoical and immunohistochemical characteristics were consistent with vascular endothelial and VSMCs respectively. The VSMCs could grow extensively on the scaffold after the in vivo implantation. The scaffold were wrapped by the fibrous tissue ten days later after the in vitro implantation of VSMCs. The seed cells grew in the scaffold, and the vessel cavity seen in the center of the scaffold, was quite different from the normal vessel structure.ConclusionIt is feasible to implant the VSMCs with fibrin gels into the living body. The vessels reconstructed, though different from the normal structure, is similar to the embryo of the vessels.

Key words: tissue engineering, blood vessels, chitosan, vascular grafts, scaffold