《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (06): 522-523.

• 基础研究 • 上一篇    下一篇

头穴丛刺法对急性脑梗死大鼠病理学及神经生长因子和转化生长因子的影响

倪金霞1a; 朱文增1b; 唐强1b; 秦颖2; 于致顺1a   

  1. 1.黑龙江中医药大学附属第二医院a.针灸科一病房;b.康复科,黑龙江哈尔滨市 150001;2.哈尔滨医科大学第一临床医学院第二住院部,黑龙江哈尔滨市 150001
  • 收稿日期:2007-02-15 出版日期:2007-06-01 发布日期:2007-06-01

Effect of Cluster Scalp Acupuncture on Pathological Changes and Expression of Nerve Growth Factor and Transforming Growth Factor in Rats after Acute Cerebral Infarction

NI Jin-xia, ZHU Wen-zeng, TANG Qiang, et al   

  1. The Second Hospital Affiliated to Heilongjiang University of Traditional Chinese Medicine, Harbin 150001, Heilongjiang, China
  • Received:2007-02-15 Published:2007-06-01 Online:2007-06-01

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摘要： 目的探讨头穴丛刺对急性脑梗死大鼠病理学及神经生长因子(NGF)和转化生长因子(TGF)的影响。方法将30只大鼠随机分为3组:假手术组(A组)、造模组(B组)、头穴丛刺组(C组),每组10只。采用线栓法制备急性脑梗死动物模型,观察头穴丛刺法对急性脑梗死大鼠病理学的影响及脑组织皮质及海马区NGF和TGF表达的变化。结果造模后第7天,B组大鼠缺血区脑组织明显水肿,周围区神经细胞数明显减少;与B组比较,C组大鼠脑缺血区脑组织水肿减轻,神经细胞数增多;术后7d,A组与B组大鼠皮质及海马区均有少量NGF及TGF表达,两组比较差异无显著性意义(P>0.05),C组大鼠皮质及海马区NGF及TGF表达明显升高,与B组比较差异有显著性意义(P<0.05,P<0.01)。结论头穴丛刺能减轻大鼠脑缺血区组织水肿,改善缺血半影区神经细胞功能状态,促进神经胶质细胞增生,并可诱导皮质及海马区NGF和TGF的表达,减轻缺血造成的神经元损伤。

关键词: 脑梗死, 大鼠, 头穴丛刺, 神经生长因子, 转化生长因子

Abstract: Objective To explore the effect of cluster scalp acupuncture on pathological changes and the expression of nerve growth factor (NGF) and transforming growth factor (TGF) in rats after acute cerebral infarction.Methods30 male Wistar rats were randomly divided into the sham operation group (group A), model group (group B) and cluster scalp acupuncture group (group C) with 10 animals in each group. Animal model was made by the middle cerebral artery occlusion. The pathological changes and the expression of NGF and TGF in the brain tissues of rats were tested.ResultsOn the 7th day after operation, brain tissue of infarction area in the group B indicated significantly edema, neural cells decreased. Compared with the group B, the edema in the group C decreased and neural cells increased. At the same time, there were a few NGF and TGF expressions in hippocampus andcortex areas of the group A and group B, and there was no difference between the two groups ( P>0.05); but compared with the group A and group B, NGF and TGF in the group C increased significantly ( P<0.05, P<0.01).ConclusionCluster scalp acupuncture therapy can decrease edema, increase neural cells in infarction areas, as well as induce the expression of NGF and TGF in cortex and hippocampus.

Key words: cerebral infarction, rats, cluster scalp acupuncture, nerve growth factor, transforming growth factor