《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (05): 438-439.

• 基础研究 • 上一篇    下一篇

平瘤康方剂对C6胶质瘤细胞增殖的抑制作用

李振飞; 张梅奎; 张笑明; 尹岭   

  1. 解放军总医院神经信息中心,北京市 100853
  • 收稿日期:2007-02-06 出版日期:2007-05-01 发布日期:2007-05-01

Inhibition of Pingliukang Medicated Serum on Proliferation of Rat C6 Glioma Cells in Vitro

LI Zhen-fei, ZHANG Mei-kui,ZHANG Xiao-ming, et al   

  1. Center of Neuroinformation, General Hospital of PLA, Beijing 100853, China
  • Received:2007-02-06 Published:2007-05-01 Online:2007-05-01

摘要: 目的探讨中药方剂平瘤康含药血清对体外培养的C6胶质瘤细胞增殖及细胞周期的影响。方法2.5%、5%、10%和20%平瘤康含药血清处理体外培养的C6胶质瘤细胞24h、48h和72h后,应用MTT比色法检测C6胶质瘤细胞增殖;PI染色后,应用流式细胞仪观测细胞周期时相。结果10%和20%的含药血清能抑制C6胶质瘤细胞的增殖;10%和20%的含药血清处理C6细胞48h、72h后,S期细胞百分率下降,呈剂量依赖性。结论平瘤康含药血清可能通过阻滞细胞周期而抑制胶质瘤细胞增殖。

关键词: 平瘤康, 血清药理学, C6胶质瘤细胞, 增殖, 细胞周期

Abstract: Objective To investigate the inhibitory effect of Pingliukang (a prescription) medicated serum on the proliferation of cultured rat C6 glioma cells and influence on cell cycle in vitro. Methods MTT colorimetry were performed to measure the levels of the proliferation of rat C6 glioma cells cultured with 2.5%, 5%, 10% and 20% of Pingliukang medicated serum for 24 h, 48 h and 72 h in vitro. The effect of Pingliukang medicated serum on cell cycle were observed by FCM. Results The proliferation of C6 cell was obviously inhibited by Pingliukang medicated serum with dose-effect relationship. The inhibitory effect of 20% of medicated serum was the strongest. When the C6 glioma cells were treated with 10% and 20% medicated serum for 48 h and 72 h, the cells of S period declined. Conclusion The Pingliukang medicated serum can inhibit the proliferation and block cell cycle of cultured C6 glioma cells.

Key words: Pingliukang prescription, serum pharmacology, C6 glioma cell, proliferation, cell cycle