《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (05): 432-433.

• 基础研究 • 上一篇    下一篇

鸦胆子油乳诱导膀胱癌细胞J82凋亡及其机制的初步研究

吕峰; 王禾; 秦卫军   

  1. 第四军医大学西京医院泌尿外科,陕西西安市 710032
  • 收稿日期:2007-03-02 出版日期:2007-05-01 发布日期:2007-05-01

Bladder Cancer J82 Cells Apoptosis Induced by Brucea Javanica Oil Emulsion and the Mechanism

Lü Feng, WANG He, QIN Wei-jun   

  1. Department of Urinary Surgery, the Xijing Hospital of the Fourth Military Medicine University, Xi'an 710032, Shaanxi,China
  • Received:2007-03-02 Published:2007-05-01 Online:2007-05-01

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被引次数

27

摘要： 目的探讨鸦胆子油乳对膀胱癌J82细胞凋亡的影响及其可能的作用机制。方法四甲基偶氮唑盐(MTT)法检测鸦胆子油乳对J82细胞增殖的抑制率;通过荧光染色、流式细胞术(FCM)检测细胞凋亡;应用caspase-3活性试剂盒检测caspase-3活性。结果在0.5～4.0ml/L浓度下,鸦胆子油乳抑制J82细胞的增殖,并呈现剂量-效应关系;鸦胆子油乳2.0ml/L作用24h后可见典型的凋亡现象;鸦胆子油乳处理组细胞样本caspase-3活性与凋亡率高于空白对照组及鸦胆子油乳加Ac-DEVD-CHO处理组。结论鸦胆子油乳能够诱导膀胱癌J82细胞凋亡,caspase-3活化参与了鸦胆子油乳诱导J82细胞凋亡的调控。

关键词: 鸦胆子, 膀胱肿瘤, 凋亡, caspase-3

Abstract: Objective To study the effect of Brucea javanica oil emulsion on apoptosis of bladder cancer J82 cells and the possible mechanism.Methods Brucea javanica oil emulsion of different concentrations (0.5～4.0 ml/L) was added to the bladder cancer J82 cell cultured in vitro.After 24 h, the proliferation of J82 cells was measured by methyl thiazole tetrazolium (MTT) assay, cell apoptosis was detected by morphological observation and flow cytometer analysis, and caspase-3 activity was assessed using a caspase-3 kit. Results 0.5～4.0 ml/L Brucea javanica oil emulsion inhibited J82 cell proliferation in a dose-dependant manner.2.0 ml/L Brucea javanica oil emulsion induced typical apoptosis 24 h after incubation. The caspase-3 activity of 2.0 ml/L Brucea javanica oil emulsion group was higher than those of control group or Brucea javanica oil emulsion plus Ac-DEVD-CHO group. Conclusion Brucea javanica oil emulsion induces J82 cell apoptosis, and this effect includes activation of caspase-3.

Key words: Brucea javanica, bladder neoplasm, apoptosis, caspase-3