《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (05): 399-400.

• 基础研究 • 上一篇    下一篇

雷帕霉素和巴曲酶复合药物涂层支架的生物相容性实验研究

陈丹; 吕安林; 张薇; 苑媛; 刁繁荣; 李军杰   

  1. 第四军医大学西京医院心血管内科 陕西西安市 710032
  • 收稿日期:2006-03-10 出版日期:2006-05-25 发布日期:2006-05-25

Biocompatibility of Rapamycin-Batroxobin Eluting Stent in Porcine Coronary Artery

CHEN Dan,Lü An-lin,ZHANG Wei, et al   

  1. Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi, China
  • Received:2006-03-10 Published:2006-05-25 Online:2006-05-25

摘要: 目的了解雷帕霉素和巴曲酶复合药物涂层支架(raPamycin-batroxobin eluting stent,RBES)的生物相容性。方法采用微喷法制备聚合物及雷帕霉素和巴曲酶复合药物涂层支架组,聚合物及雷帕霉素涂层支架作为对照组。支架置入小型猪冠状动脉,每组8只猪,每只猪置入上述1枚支架。支架置入前、后即刻及终点时行冠状动脉造影。分析支架置入前及终点时的全血细胞计数、生化指标、血压、心率、体重、全程的行为状态;处死动物后取出支架置入段冠状动脉,行扫描电子显微镜观察及组织学观察测量;取肺、胃、小肠、大肠、肝、肾以及支架血管段供血的心室壁行组织学观察。结果两组均未发现骨髓抑制、溶血、对肝、肾功能及代谢无明显影响,两组中心率、血压、体重的变化差异无显著性,对行为状态无影响。结果所有支架置入成功。实验期(28d)内两组中冠状动脉无狭窄,内膜面积及内膜面积与中膜面积的比率两组间无显著性差异,未见明确的炎症反应细胞。未发现肺、胃、小肠、大肠、肝、肾以及支架血管段供血的心室壁与RBES涂层支架有关的病理损害。结论RBES置入小型猪冠状动脉后28d的血液相容性及组织相容性良好。

关键词: 支架, 组织相容性, 雷帕霉素, 巴曲酶

Abstract: ObjectiveTo illustrate the biocompatibility of rapamycin-batroxobin eluting stent(RBES). MethodsStent made with rapamycin-batroxobin and polymer were implanted in the coronary artery of experimental group and the rapamycin with the polymer in the control group, which including 8 miniature pigs in each group. Coronary arteriographies were taken before, exactly after implanting the stent and before sacrificed the pigs. The whole blood cell counts, biochemical indexes, blood pressures, heart rates, body weights before implantation and sacrifice, together with the behavior states during the observation were analyzed. The coronary arteries of the implantation segment were observed under the scanning electron microscope and histologically. Lungs, stomachs, intestines, bowels, livers, kidneys and the ventricular walls which infused by the stents supported arteries were also observed histologically. ResultsBone marrow suppression, hemolysis, liver and kidney disfunction had not been found in the 2 groups. There was no significant difference in heart rate, blood pressure and body weight between 2 groups. Behavior was not influenced. All stents were implanted successfully. And there was no stenosis in coronary artery in both group during the 28-day period. There were no significant differences in endothelium square and the ratio of endothelium square to tunica media square. No definite inflammatory cell was found. There were no pathological damages related to RBES in lungs, stomachs, intestines, bowels, livers, kidneys and the ventricular walls infused by the stent supported arteries.ConclusionThe blood compatibility and histocompatibility were good after RBES were implanted in coronary arteries of miniature pigs.

Key words: stent, histompatibility, rapamycin, batroxobin