《中国康复理论与实践》

《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (05): 388-390.

• 基础研究 • 上一篇    下一篇

脊髓损伤后诱导异位骨化实验模型

任宪锋1,2; 关骅1,2; 王晖3; 王晶晶3   

  1. 1.首都医科大学康复医学院 北京市 100068;2.北京博爱医院脊柱脊髓外科 北京市 100068;3.首都医科大学实验动物科学部 北京市 100054
  • 收稿日期:2006-04-05 出版日期:2006-05-25 发布日期:2006-05-25

Induction of Heterotopic Ossification in Rabbits after Spinal Cord Injury

REN Xian-feng, GUAN Hua, WANG Hui, et al   

  1. Faculty of Rehabilitation of Capital University of Medical Sciences, Beijing 100068, China
  • Received:2006-04-05 Published:2006-05-25 Online:2006-05-25

PDF (PC)

被引次数

27

摘要： 目的建立一种脊髓损伤后诱导异位骨化的方法。方法10只新西兰白兔胸10水平脊髓完全横断后,右后肢膝关节呈伸直位石膏固定5周,每天去除石膏连续被动屈伸膝关节,每周6次,每次5min。观察局部皮肤温度、肢体肿胀度及骨化情况;第5周末将兔处死,取骨化处行组织学检查。结果10只新西兰兔均成功诱导出异位骨化。新西兰兔右后肢肿胀明显,局部皮肤温度升高。诱导的异位骨化与临床上观察到的异位骨化组织学表现一致。结论被动活动+固定法能成功诱导异位骨化,可以用来探讨脊髓损伤后异位骨化的病理机制。

关键词: 脊髓损伤, 异位骨化, 骨化性肌炎, 动物模型, 兔

Abstract: ObjectiveTo establish a method to induce heterotopic ossification (HO) after spinal cord injury. Methods10 New Zealand White rabbits underwent a complete transection of the thoracic (T10) spinal cord. Just after transection, the right hind limb of every rabbit was immobilized with a plaster support to keep the knee in extension and the hip unrestricted. The plaster was temporarily removed once a day (six times per week) to allow the knees passively mobilized at the maximum range for 5 min. Local swelling, local skin temperature and grade of ossification of the paraplegic limbs were observed. After 5 weeks, the rabbits were sacrificed, and the quadriceps muscles of the right hind limbs were removed for the histological examination. ResultsAfter 5-week immobilization and temporary passive mobilization, HO was successfully induced in all 10 rabbits. There was local skin temperature increase and local swelling in the right hind limbs. The histological changes were similar to those observed in clinical HO. Conclusion5-week immobilization and temporary passive mobilization approach may successfully induce HO, and it may be used to study the pathogenesis of HO after spinal cord injury.

Key words: spinal cord injury, heterotopic ossification, myositis ossificans, animal model, rabbit