《中国康复理论与实践》

《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (04): 304-306.

• 基础研究 • 上一篇    下一篇

骨髓细胞胸腺内注射对大鼠小肠移植急性排斥反应的影响

罗长江; 王为忠; 李纪鹏; 陈冬利; 董光龙; 刘骥   

  1. 第四军医大学西京医院胃肠外科 陕西西安市 710032
  • 收稿日期:2005-11-21 出版日期:2006-04-25 发布日期:2006-04-25

Effect of Intrathymic Injection of Allogene Bone Marrow on Small Intestine Transplantation of Rats

LUO Chang-jiang, WANG Wei-zhong, LI Ji-peng, et al   

  1. The Department of Gastrointestinal Surgery, Xijing Hospital, the Fourth Military Medical Universi- ty, Xi'an 710032, Shaanxi, China
  • Received:2005-11-21 Published:2006-04-25 Online:2006-04-25

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摘要： 目的了解异基因骨髓注射在大鼠小肠移植中的免疫耐受作用和意义。方法选用近交系大鼠F344/N和Wistar/A进行全小肠异位移植,试验组在行异基因移植前7d取供体骨髓细胞(BMC)行受体胸腺内注入,对照单纯同基因及异基因大鼠移植模型了解异基因供体骨髓在受体胸腺内注射能否减少移植后急性排斥反应的发生。结果异基因大鼠异位全小肠移植术后第3、5、7天可出现典型的轻、中、重度排斥反应,而同基因组和试验组未出现排斥反应。异基因移植组术后3d血清可溶性白细胞介素-2受体(sIL-2R)及肿瘤坏死因子-α(TNF-α)水平明显高于其他组(P<0.01),且随排斥反应的加重,进一步增高。而异基因骨髓胸腺内注射组血清sIL-2R及TNF-α水平仅在第3、5天轻度升高,并呈下降趋势,与同基因移植组无显著性差异(P>0.05)。结论移植术前7d异基因供体骨髓在受体胸腺内注射能明显减少小肠移植后急性排斥反应的发生;血清sIL-2R和TNF-α检测有可能作为早期诊断小肠移植急性排斥反应的敏感免疫指标。

关键词: 胸腺内注射, 骨髓细胞, 小肠移植, 免疫耐受, 可溶性白细胞介素-2受体, 肿瘤坏死因子-α

Abstract: ObjectiveTo investigate the immune tolerance function and significance of allogene bone marrow injection to the small intestines transplantation of rats.MethodsInbreeding line rat F344/N and Wistar/A were selected to perform heterotopic graft of the whole small intestine. 7 days before allogene transplantation, donator bone marrow cells (BMC) were injected into thymus of acceptor (the testing group). According to the isogene and allogene rat transplant model, it was comprehended whether injecting allogene donator marrow into acceptor thymus could decrease the acute rejection after transplantation.Results3, 5 or 7 days after allogeneic rat dystopia whole small intestine transplantation, typical reject reaction appeared, but there was no reject reaction in isogenome and testing group. 3 days after allotransplantation, serum soluble interleukin-2 receptor (sIL-2R) and tumor necrotic factor-α (TNF-α) levels were significantly higher than the other groups (P<0.01). The level of serum sIL-2R and TNF-α in the allogene marrow injecting group were only slight higher on the 3rd or 5th day, and getting downtrend, and there was no significant difference compared with isogenic transplantation group.ConclusionAllogenic donator bone marrow intrathymic injecting into acceptor 7 days before small intestina transplantation, can reduce the reject reaction after the grafting. The levels of serum sIL-2R and TNF-α can be selected as a sensitive early diagnosis index of acute rejection after small intestine transplantation.

Key words: intrathymic injection, bone marrow cell, small intestine transplantation, immune tolerance, soluble interleukin-2 receptor (sIL-2R), tumor necrotic factor-α (TNF-α)