《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (03): 255-257.

• 基础研究 • 上一篇    下一篇

解毒化瘀Ⅱ方对急性肝衰竭大鼠肝细胞Bcl-2及细胞色素C表达的影响

毛德文; 邱华; 农朝赞; 刘洁; 石清兰   

  1. 广西中医学院第一附属医院中医肝病治疗中心,广西南宁市 530023
  • 收稿日期:2006-11-20 出版日期:2007-03-01 发布日期:2007-03-01

Effect of the Jiedu Huayu Ⅱ Decoction on Mitochondrial Bcl-2 and Cytochrome C Expression in Liver in Rats with Acute Hepatic Failure

MAO De-wen, QIUHua, NONG Chao-zan, et al.   

  1. No.1Hospital Affiliated to Guangxi Chinese Medical University, Nanning 530023, Guangxi, China
  • Received:2006-11-20 Published:2007-03-01 Online:2007-03-01

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摘要： 目的通过观察解毒化瘀Ⅱ方对急性肝衰竭大鼠肝线粒体Bcl-2、细胞色素C表达的影响,探讨其抗肝衰竭的作用机制。方法以硫代乙酰胺(TAA)皮下注射复制急性肝衰竭大鼠模型,SPF级Wistar大鼠84只,随机分为空白组,模型组,解毒化瘀Ⅱ方低、中、高剂量组,安宫牛黄丸组,乳果糖组;造模前3d开始灌胃给药,共给药5.5d;采用WesternBlot法检测各组大鼠肝细胞线粒体Bcl-2表达及细胞色素C在肝线粒体和细胞质中的表达情况。结果与空白组比较,模型组大鼠肝线粒体内Bcl-2表达强度显著降低,细胞色素C在细胞质中的表达增强,在线粒体中的表达减弱;解毒化瘀Ⅱ方能提高急性肝衰竭大鼠肝线粒体内Bcl-2的表达,抑制细胞色素C从肝线粒体释放到细胞质中,并呈现剂量-效应关系。结论解毒化瘀Ⅱ方对暴发性肝衰竭大鼠肝细胞的保护作用机制有可能是通过增强肝细胞线粒体Bcl-2表达,抑制肝线粒体膜通透性转换孔开放,阻止细胞色素C释放到细胞质,抑制肝细胞凋亡的发生。

关键词: 解毒化瘀Ⅱ方, 急性肝衰竭, 线粒体, Bcl-2, 细胞色素C, 大鼠

Abstract: Objective To explore the mechanism of the Jiedu Huayu Ⅱ Decoction in actue hepatic failure.MethodsThe acute hepatic failure rat models were induced with thioacetamide (TAA).84 SPF Wistar rats were divided into 7 groups randomly:blank group,model group,low dose group,middle dose group,high dose group,lactulose group,and the Angong Niuhuang Pill group.The drugs were administrated by gavage 3 d before model-making,5.5 d in total.The expression of Bcl-2,cytochrome C in mitochondrial and cytoplasm were measured with Western Blot.ResultsThe expression of Bcl-2 reduced in mitochondrial,the expression of cytochrome C increased in cytoplasm and reduce in mitochondrial in model group compare with that of blank group.Jiedu Huayu Ⅱ Decoction was able to enhance the expression of Bcl-2 and restrain cytochrome C release from mitochondrial to cytoplasm.Moreover,it shows dose-effect relation.ConclusionThe protection of Jiedu Huayu Ⅱ Decoction for hepatocyte in acute hepatic failure rats maybe relate to the increase of Bcl-2 in mitochondrial,blocking the mitochondrial permeability transition pore to arrest cytochrome C releasing.

Key words: Jiedu Huayu Ⅱ Decoction, acute hepatic failure, mitochondria, Bcl-2, cytochrome C, rats