《中国康复理论与实践》

《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (02): 131-132.

• 基础研究 • 上一篇    下一篇

亚硒酸钠拮抗庆大霉素所致豚鼠耳蜗毒性实验研究

高良才1; 李唐英1; 路虹2; 唐立滨1; 张立杰1   

  1. 1.唐山市人民医院耳鼻咽喉科 河北唐山市 063001;2.河北医科大学第二附属医院耳鼻咽喉科 河北石家庄市 050017
  • 收稿日期:2005-01-17 出版日期:2006-02-25 发布日期:2006-02-25

Experimental Study of Antitoxic Effect of Sodium Selenite on Guinea Pig Cochlea Injury Induced by Gentamicin in Vivo

GAO Liang-cai, LI Tang-ying, LU Hong, et al   

  1. The Department of Otolaryngology, People's Hospital of Tangshan, Tangshan 063001,Hebei, China
  • Received:2005-01-17 Published:2006-02-25 Online:2006-02-25

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被引次数

1

摘要： 目的观察亚硒酸钠对庆大霉素所致活体豚鼠耳蜗损害是否具有拮抗作用。方法20只豚鼠随机分为实验组和对照组各10只,给予肌肉注射庆大霉素200mg/kg/d,共8d;实验组同时口服亚硒酸钠1mg/kg/d,共8d。用药前后进行听性脑干反应(ABR)测试观察听功能变化;通过扫描电镜(SEM)及透射电镜(TEM),观察耳蜗形态学变化。结果对照组动物的ABR阈值比实验组高约30dB(P<0.01),Ⅰ波潜伏期较实验组延长约0.23ms(P<0.01);SEM示对照组动物耳蜗外毛细胞纤毛区域性缺失;实验组纤毛形态基本正常;TEM示对照组动物耳蜗外毛细胞线粒体外膜破坏,局部膨出,次级溶酶体增多,可见髓样小体;实验组动物耳蜗外毛细胞基本正常。结论亚硒酸钠对庆大霉素所致活体豚鼠耳蜗损伤有拮抗作用。

关键词: 亚硒酸钠, 庆大霉素, 耳毒性, 扫描电镜, 透射电镜

Abstract: ObjectiveTo explore the effect of sodium selenite on gentamicin-induced ototoxicity.MethodsTwenty guinea pigs were randomly divided into the experimental group and control group with 10 animals in each group. The animals were treated with i.m injection of gentamicin (200 mg/kg/d for 8 consecutive days, and at same time, the experimental group was added with p.o tablet of sodium selenite (1 mg/kg/d). Before and after the administration, hearing function was evaluated by examination for auditory brain stem responses (ABR). The cochlear outer haircells were observed by scanning electronic microscope (SEM) and transmission electronic microscope (TEM).ResultsABR threshold of the control group was about 30 dB above that of the experimental group (P<0.01). The latency of waveⅠof the control group was about 0.23 ms above that of the experimental group (P<0.01). Under SEM, the cilia of the majority outer hair cells of the control group lodged even disappeared, while that of the experimental remained regular. Under TEM, in outer hair cells of the control group, mitochondrial crests were obscure, out-membrane was damaged and local protruding, the number of secondary lysosomes was increased, myeloid bodies appeared, but in the experimental group, outer hair cells basically remained normal.ConclusionSodium selenite has antitoxic effect on guinea pig cochlea injury induced by gentamicin in vivo.

Key words: sodium selenite, gentamicin, ototoxicity, scanning electron microscopy, transmission electron microscopy