《中国康复理论与实践》

《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (01): 35-36.

• 基础研究 • 上一篇    下一篇

脊髓损伤后白细胞介素-1β、caspase-3的表达和细胞凋亡的相关性

王鸿飞; 郑连杰; 杨梁   

  1. 大连医科大学第二附属医院 辽宁大连市 116023
  • 收稿日期:2005-10-08 出版日期:2006-01-25 发布日期:2006-01-25

Correlation among apoptosis, expression of interleukin-1β and caspase-3 after spinal cord injury in rats

WANG Hong-fei, ZHENG Lian-jie, YANG Liang   

  1. the Second Affiliated Hospital of Dalian Medical University, Dalian 116023, Liaoning, China.
  • Received:2005-10-08 Published:2006-01-25 Online:2006-01-25

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摘要： 目的研究大鼠脊髓损伤后白细胞介素-1β(IL-1β)、casPase-3表达和细胞凋亡的规律及三者之间的关系。方法成年SD大鼠随机分为假手术组和损伤组,于伤后1h、8h、24h、72h处死,用免疫组化染色检测IL-1β、casPase-3阳性细胞,TUNEL法标记凋亡细胞。结果假手术组仅表达少量IL-1β和casPase-3阳性细胞;损伤组两者均在8h表达最多,24h减少,72h减少至略多于假手术组水平。TUNEL标记阳性细胞的时间分布特点与IL-1β和casPase-3相似;IL-1β、casPase-3阳性细胞率和细胞凋亡指数三者间存在正相关(P<0.01)。结论大鼠脊髓损伤后,IL-1β和casPase-3表达增强,凋亡细胞大量出现,三者间存在正相关。

关键词: 脊髓损伤, 凋亡, 白细胞介素-1β(IL-1β), caspase-3

Abstract: ObjectiveTo study the characteristic of apoptosis and the expression of interleukin-1β(IL-1β)and caspase-3, as well as the relation among them after spinal cord injury(SCI) in rats.MethodsAdult SD rats were divided into two groups randomly: sham group and spinal cord injury group. The rats were killed at 1 h, 8 h, 24 h, and 72h after surgery. The expression of IL-1β and caspase-3 was determined with immunohistochemistry and the apoptosis was labeled by TUNEL.ResultsThere was only a few cells that expressed low level IL-1β and caspase-3 in sham group, but they both enhanced in SCI group within 8 h after operation, and then decreased to the level similar to sham group 72 h after operation. The rates of TUNEL-positive cells were similar with that of IL-1β and caspase-3 after SCI. There was positive correlation among expression of IL-1β and caspase-3 and apoptosis index.ConclusionExpression of IL-1β and caspase-3 increase and a large number of TUNEL-positive cells present after SCI in rats, and there is positive correlation among them.

Key words: spinal cord injuries, apoptosis, interleukin-1β(IL-1β), caspase-3