《中国康复理论与实践》

《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (01): 11-13.

• 专题 • 上一篇    下一篇

微创开颅法局灶性大鼠脑梗死模型的实验研究

薛静; 高培毅; 安沂华   

  1. 首都医科大学附属北京天坛医院神经内科 北京市 100050
  • 收稿日期:2005-09-16 出版日期:2006-01-25 发布日期:2006-01-25

A Model of Focal Cortical Infarctionin Rat:Mini mally Invasive Craniotomy

XUE Jing,GAO Pei-yi,AN Yi-hua,SUN Chong-ran,LI Jin,HUANG Hua   

  1. The Department of Neuroradiology, the Affiliated Beijing Tiantan Hospital of Capital University of Medical Sciences, Beijing 100050, China
  • Received:2005-09-16 Published:2006-01-25 Online:2006-01-25

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被引次数

11

摘要： 目的建立一种稳定的大鼠脑局灶性梗死模型,以用于脑梗死后神经干细胞移植的长期观察。方法37只大鼠随机分为实验组和对照组。微创法经颞骨局部钻孔开颅,采用直接结扎大脑中动脉终段,同时永久结扎同侧颈总动脉、暂时性夹闭对侧颈总动脉的方法制备大鼠脑梗死模型。通过大鼠脑梗死后的神经功能评分、墨汁灌注、TTC染色、MRI成像结果对该模型进行评价。结果大鼠术后状态良好,实验组大鼠观察4周后死亡率低为6.25%。大鼠神经功能评分均为1分,墨汁灌注及TTC染色观察梗死范围局限于皮层,4周后MRI成像测量梗死体积稳定,平均为83.52 mm3。结论该模型对大鼠创伤小,梗死灶的位置和体积恒定,长期存活率高,为脑梗死后神经干细胞移植的研究提供了一种理想的动物模型。

关键词: 脑梗死, 动物模型, 大脑中动脉, 大鼠

Abstract: ObjectiveTo develop a stable model of focal cerebral infarction in rat to study the curative effect of neural stem cells transplantation.MethodsThirty-seven rats were selected which were divided into two groups in random, experimental group and control group. The focal infarction model was developed by the ligation of the left middle cerebral artery followed by the ligation of the ipsilateral common carotid artery and the temporary clip occlusion of the contralateral common carotid artery for 1.5 h. The operation adopted minimally invasive craniotomy though temporal bone. The model was evaluated by examining the neurologic deficits, ink perfusion, TTC staining and Magnetic Resonance imaging.ResultsAll the rats were in good condition after the operation, the mortality rate was 6.25% after 4 weeks. Ink perfusion and TTC staining confirmed that the ischemia was confined to the cortex. The areas of infarction measured 83.52 mm3 by Magnetic Resonance imaging after 4 weeks.ConclusionA stable focal cerebral infarction model can be achieved by minimally invasive craniotomy. It is superior for its homogeneity of infarction volume and site, and its low mortality. It can be used for the study of transplantation of neural stem cells.

Key words: cerebral infarction, animal model, middle cerebral artery, rat