《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (11): 919-921.

• 基础研究 • 上一篇    下一篇

关节腔注射几丁糖对兔关节软骨退变的影响

王志林; 刘世清; 陶海鹰; 李章华   

  1. 武汉大学人民医院骨科 湖北武汉市 430060
  • 收稿日期:2005-05-08 出版日期:2005-11-25 发布日期:2005-11-25

Effects of chitosan injected intra-articular on cartilage degeneration of rabbits

WANG Zhi-lin, LIU Shi-qing, TAO Hai-ying, et al   

  1. Department of Orthopaedic, The People's Hospital of Wuhan University, Wuhan 430060, Hubei, China
  • Received:2005-05-08 Published:2005-11-25 Online:2005-11-25

摘要: 目的观察关节腔注射几丁糖对兔关节软骨退变的影响。方法18只大白兔行右膝关节前交叉韧带切断术建立骨关节炎(OA)动物模型。术后将动物随机分为2组,每组9只,实验组行2%几丁糖0.3 ml关节腔注射,每2周1次,对照组同一时间点给予生理盐水0.3 ml关节腔注射。第8周处死动物,比较两组兔股骨髁关节软骨的大体改变,用生化方法测定软骨基质蛋白多糖的变化,并用免疫组化方法对比两组的基质金属蛋白酶-1(MMP-1)的表达情况。结果实验组动物软骨退变程度轻于对照组(P<0.05),氨基葡聚糖总量明显高于对照组(P<0.01),硫酸软骨素/硫酸角质素比值低于对照组(P<0.05),MMP-1的表达亦明显低于对照组(P<0.01)。结论几丁糖能预防OA软骨退变,具有软骨保护作用。

关键词: 几丁糖, 软骨, 骨关节炎

Abstract: ObjectiveTo observe the effects of chitosan injected intra-articular on cartilage degeneration of rabbits with osteoarthritis(OA).MethodsOA animal model was established by unilateral anterior cruciate ligament transaction(ACLT) in 18 rabbits.After operation,animals were randomly divided into the experimental group and control group with 9 rabbits in each group.The experimental group was treated with intra-articular injection of 0.3 ml of 2% chitosan every two weeks after operation.While,the control group was injected with 0.3 ml normal saline at the same time.All rabbits were killed at the 8th week.Cartilage degradation of femoral condyles was evaluated at macroscopic level and the changes of proteoglycan of articular cartilage matrix were detected by biochemical technique,and the expression of matrix metalloproteinases-1(MMP-1) was documented by immunohistochemistry.ResultsCartilage degradation in the control group was significantly more severe than that in the experimental group in macroscopic grading scale(P<0.05).The detection of proteoglycan showed that the quantity of glycosaminoglycans increased(P<0.01) and the chondroitin sulfate/keretan sulfate declined(P<0.05) in experimental group compared with that in the control group.Immunohistochemistry study showed that the expression of MMP-1 in the control group was significantly more severe than that in the experimental group(P<0.01).ConclusionChitosan can prevent cartilage degaradation and protect cartilage.

Key words: chitosan, cartilage, osteoarthritis