《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (11): 1005-1006.

• 专题　神经泌尿康复 • 上一篇    下一篇

硝酸甘油对脊髓损伤雌性大鼠离体尿道肌条张力的影响

鞠彦合1,2; 廖利民1,2; 叶云龙1,2; 吕振1,2   

  1. 1.首都医科大学康复医学院,北京市 100068;2.中国康复研究中心北京博爱医院泌尿外科,北京市 100068
  • 收稿日期:2008-11-05 出版日期:2008-11-01 发布日期:2008-11-01

Effects of Nitroglycerin on Urethral Sphincter Strips Tension of Chronic Complete Spinal Cord Injured Female Rats in Vitro

JU Yan-he, LIAO Li-min, YE Yun-long,et al   

  1. Capital Medical University School of Rehabilitation Medicine, Department of Urology, Beijing Charity Hospital, China Rehabilitation Research Centre, Beijing 100068, China
  • Received:2008-11-05 Published:2008-11-01 Online:2008-11-01

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摘要： 目的研究一氧化氮(NO)供体硝酸甘油对慢性期完全性脊髓损伤雌鼠离体尿道肌条张力的影响。方法3月龄健康雌性未育SD大鼠30只,手术横断胸10脊髓。饲养8周后,筛选出BBB评分≤5分的大鼠10只作为实验组,随机选取10只正常鼠作为对照组,测定组织浴槽中加入硝酸甘油0.5mg前后,尿道肌条近段平滑肌部分和远段外括约肌复合体部分张力的变化。结果组织浴槽中加入硝酸甘油0.5mg后,正常鼠尿道肌条远段张力值从(640.50±91.79)mg降低至(597.50±92.61)mg,实验鼠尿道肌条远段张力值从(485.50±68.94)mg降低至(459.90±69.51)mg。正常鼠尿道肌条近段、远段用药前后张力差分别为(222.30±12.14)mg和(42.00±7.86)mg;实验鼠尿道肌条近段、远段用药前后张力差分别为(223.00±15.78)mg和(25.60±5.17)mg。结论组织浴槽中加入硝酸甘油对尿道平滑肌和尿道外括约肌复合体均产生松弛效应,对正常鼠尿道外括约肌复合体产生的松弛效应大于脊髓损伤鼠,但对尿道平滑肌产生的松弛效应大致相同。

关键词: 脊髓损伤, 尿道平滑肌, 尿道外括约肌, 硝酸甘油, 一氧化氮, 大鼠

Abstract: Objective To study the effects of nitroglycerin on urethral sphincter of chronic complete spinal cord injury female rats in vitro. Methods 30 SD female rats with 3 months old were transected at T10 spinal cord. After 8 weeks, the 10 rats with BBB scores ≤5 were selected as experimental group, and other 10 normal rats as control group. The difference of tension in both proximal segment (urethral smooth muscle strips) and distal segment (external urethral sphincter muscle strips) were surveyed before and after nitroglycerin 0.5 mg administration in bath. Results The tension of distal segment in control group reduced from (640.50±91.79) mg to (597.50±92.61) mg, and from (485.50±68.94) mg to (459.90±69.51) mg in experimental group. The difference between proximal segment and distal segment were (222.30±12.14) mg and (42.00±7.86) mg before and after administration in control group, and were (223.00±15.78) mg and (25.60±5.17) mg in experimental group. Conclusion The administration of nitroglycerin 0.5 mg in bath can reduce the tension of both urethral smooth muscle and external urethral sphincter in both normal and spinal cord injured rats. The relaxative effect on external urethral sphincter muscle of normal rats exceed that of injured ones, but approximate on urethral smooth muscle.

Key words: spinal cord injury, urethral smooth muscle, external urethral sphincter, nitroglycerin, nitric oxide, rats