《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (10): 930-931.

• 基础研究 • 上一篇    下一篇

寰枢椎周围软组织损伤对小鼠记忆能力的影响

朱彤彤1a; 韩向北1b,2; 郭亚雄1b; 李德龙1b; 赵丽娟1b   

  1. 1.吉林大学白求恩医学院a.2006级临床医学七年制;b.病理生理教研室,吉林长春市 130021;2.吉林省人民医院内分泌科,吉林长春市 130021
  • 收稿日期:2007-12-03 出版日期:2008-10-01 发布日期:2008-10-01

Effect of Soft Tissues Injury around Atlantoaxial Joint on Memory Abilities of Mice

ZHU Tong-tong, HAN Xiang-bei, GUO Ya-xiong, et al   

  1. The Department of Pathophysiology, School of Bethune Medical Sciences, Jilin University, Changchun 130021,Jilin, China
  • Received:2007-12-03 Published:2008-10-01 Online:2008-10-01

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摘要： 目的观察寰枢椎周围软组织损伤对小鼠学习记忆能力的影响。方法44只小鼠随机分为模型组和对照组,各22只。模型组小鼠利用手术方法破坏寰枢椎周围软组织建立颈椎稳定性异常病理模型;采用水迷宫试验、跳台试验和洞板试验测试其学习、记忆能力;HE染色观察脑海马细胞数量;免疫组织化学染色观察脑海马caspase-3的表达。结果与对照组相比,模型组小鼠水迷宫试验中游全程时间和错误次数明显增加(P<0.01);跳台试验中模型组小鼠反应期明显延长,潜伏期明显缩短,错误次数明显增加(P<0.01);洞板试验中模型组小鼠潜伏期延长,探洞次数明显减少(P<0.01)。HE染色模型组小鼠脑海马可见明显散在的或连续的神经细胞变性、死亡;免疫组织化学染色显示模型组小鼠脑海马caspase-3蛋白呈阳性表达。结论寰枢椎周围软组织损伤可致颈椎稳定性异常而促进小鼠脑细胞凋亡,导致学习、记忆功能明显减退。

关键词: 软组织, 学习记忆, 寰枢椎关节

Abstract: Objective To observe the effect of the soft tissues injury around atlantoaxial joint on memory abilities of mice.Methods 44 mice were divided into the model group and control group with 22 animals in each group.The soft tissues around atlantoaxial joint were injured in the model group by surgery between the first and the second cervical vertebrae.The changes of learning and memory of mice were observed with step-down avoidance test,water-maze test and hole-board test;the expression of caspase-3 in hippocampus was observed by immunohistochemical staining.Results Compared with the control group,escape latency was longer and wrong times were more in water-maze test of the model group(P<0.01). In step-down avoidance test of the model group,the reaction period prolonged and wrong times increased obviously(P<0.01).In hole-board test,the latency period prolonged and the times of extending the holes decreased significantly(P<0.01).Degenerated or dead neuron in hippocampus of surgery-treated mice was found scatteredly or continuously with HE staining,the expression of caspase-3 protein was showed positive with immunohistochemical staining.Conclusion The soft tissues injury arround atlantoaxial joint can induce abnormality of the stability of cervical vertebra,which aggravated the aging process of the mice and decreased abilities of learning and memory.

Key words: soft tissue, learning and memory, atlantoaxial joint