《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (07): 552-552.

• 基础研究 • 上一篇    下一篇

一氧化氮对骨肉瘤细胞表面CD44表达的影响

张新宇; 匡正达; 杨传铎   

  1. 武警总医院骨科 北京市 100039
  • 收稿日期:2005-01-13 出版日期:2005-07-25 发布日期:2005-07-25

Effect of nitric oxide on expression of CD44 of osteosarcoma cell line

ZHANG Xin-yu, KUANG Zheng-da, YANG Chuan-duo   

  1. Department of Orthopaedics, General Hospital of Chinese People's Armed Police Forces, Beijing 100039, China
  • Received:2005-01-13 Published:2005-07-25 Online:2005-07-25

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摘要： 目的研究一氧化氮(NO)对体外培养的骨肉瘤细胞株表面CD44表达的影响及意义。方法在体外培养骨肉瘤细胞株培养液中加入不同浓度的硝普钠(SNP)以产生外源性NO,用免疫组织化学的方法研究细胞表面CD44抗原成分的变化。结果肿瘤细胞表面CD44抗原在SNP存在的情况下表达明显下降。结论NO可以一定程度上抑制骨肉瘤细胞株CD44的表达,可能引起肿瘤细胞转移能力的改变。

关键词: 一氧化氮, 骨肉瘤, 硝普钠, CD44

Abstract: ObjectiveTo study the influence of nitric oxide on the expression of CD44 of human osteosarcoma cell line (HOS).MethodsDifferent concentrations of sodium nitroprusside (SNP) were added into the culture solution of HOS in vitro. Through immunohistochemistry method (SP), the expression of CD44 were observed.ResultsThe expression of CD44 was depressed when the concentration of SNP was increased. ConclusionNitric oxide can depress the expression of CD44 of HOS and maybe change the invasive or metastatic abilities of HOS.

Key words: nitric oxide, Osteosarcoma, sodium nitroprusside, CD44