《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (07): 548-549.

• 基础研究 • 上一篇    下一篇

两种新生儿缺血缺氧性脑病模型建模方法的比较

骆健明; 庄明华; 刘明发; 白晔   

  1. 汕头大学医学院第二附属医院神经外科 广东汕头市 515041
  • 收稿日期:2005-02-02 出版日期:2005-07-25 发布日期:2005-07-25

Comparison of two methods of building the hypoxic-ischemic encephalopathy model

LUO jian-ming, ZHUANG ming-hua, LIU ming-fa, et al   

  1. Department of Neurosurgery, Second Affiliated Hospital, Shantou University Medical College, Shantou 515041, Guangdong, China
  • Received:2005-02-02 Published:2005-07-25 Online:2005-07-25

PDF (PC)

摘要： 目的比较两种新生儿缺血缺氧性脑病(HIE)模型建模方法的差异,探讨麻醉和动物缺血缺氧间期对HIE动物模型的影响。方法采用7d龄SD乳鼠建立HIE模型。传统组动物乙醚麻醉后结扎左侧颈总动脉,休息2～3h,同时入37℃水溶闭缺氧箱,持续充以8%氧+92%氮的混合气体,气流量1L/min,维持2.5h。改进组动物动脉结扎手术前动物不予麻醉,且术后动物不作休息立即进行缺氧。比较建模过程中两组动物的存活情况;光镜下观察建模后3d两组动物左侧大脑半球的病理变化情况,并比较两组动物病理总评分的差异。结果改进组建模过程中所有动物未见死亡,传统组建模过程中6只动物死亡(P<0.025)。建模后3d,改进组动物脑组织变性、坏死显著,病理总评分550分,传统组大部分动物脑组织未见明显病变,总评分130分(P<0.01)。结论传统HIE建模方法动物在建模过程中死亡率高,而存活的模型动物病理变化却不确切。改进的HIE建模方法模型动物个体之间均衡性好,脑部病变确切,建模过程中动物死亡率低,值得推广。

关键词: 缺氧性脑病, 动物模型, 建模, 大鼠

Abstract: ObjectiveTo compare the difference between the two methods in building the hypoxic-ischemic encephalopathy model, and to investigate the effect of anaesthesia and the hypoxic-ischemic intermission on the HIE model.Methods7-day-postnatal SD rats were used to build the HIE models. In the traditional group, rats were exposed to 8% O2 for 2h 2～3 h after left carotid artery ligation followed aether anaesthesia. However, rats in the improved group received hypoxia immediately after operation without previous anaesthesia. The quantity of the dead rats in two groups was counted. The morphologic changes in the brain 3 days after the hypoxic-ischemic insult were observed, and the grades of the total brain pathological evaluations of two groups were compared.ResultsNone of the animals died in the improved group, but 6 died in the traditional group. Remarkable degeneration or necrosis was detected in the ipsilateral cerebral cortex and hippocampus in the improved group, but not detected in most of the rats in the traditional group. The total grade of brain pathological evaluation in the traditional group was 130, significantly lower than that in the improved group, which was 550(P<0.01).ConclusionThe improved approach show the advantage of low mortality and credible pathological change to build the HIE model compare with the traditional methods.

Key words: hypoxic-ischemic encephalopathy, animal model, modeling, rat