《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (07): 509-511.

• 专题 • 上一篇    下一篇

吡咯喹啉醌对NMDA诱发大鼠海马神经元损伤的影响

熊顺华1; 冯桂香2; 李链1; 王星文2   

  1. 1.郧阳医学院生理教研室 河北十堰市 442000;2.郧阳医学院机能实验室 湖北十堰市 442000
  • 收稿日期:2005-02-28 出版日期:2005-07-25 发布日期:2005-07-25

Protective effect of pyrroloquinoline quinone on damage of hippocampal neurons induced by NMDA in rats

XIONG Shun-hua,FENG Gui xiang, LI Lian, et al   

  1. Department of Physiology, Yunyang Medical College, Shiyan 442000, Hubei, China
  • Received:2005-02-28 Published:2005-07-25 Online:2005-07-25

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摘要： 目的探讨吡咯喹啉醌(PQQ)对N-甲基-D-天冬氨酸(NMDA)诱发海马神经元损伤的影响。方法体外原代培养新生大鼠海马神经元,毒性剂量的NMDA诱致海马神经元损伤,预加PQQ,镜下观察神经元的形态变化,琼脂糖凝胶电泳观察细胞的死亡情况,激光共聚焦显微镜观察细胞内Ca2+浓度的变化。结果体外培养8d的海马神经用0.1mmol/LNMDA处理后,细胞内Ca2+快速增加,细胞以坏死和凋亡两种形式死亡,细胞的存活率降低;预先给予PQQ可明显减少细胞内Ca2+的增加,减少细胞死亡。结论PQQ对NMDA诱发的海马神经元损伤具有保护作用。

关键词: 吡咯喹啉醌(PQQ), 海马神经元, N-甲基-D-天冬氨酸(NMDA), 大鼠

Abstract: ObjectiveTo investigate the protective effect of pyrroloquinoline quinone (PQQ) on damage of hippocampal neurons induced by NMDA.MethodsHippocampal neurons were cultured in vitro and NMDA was used to induce neurotoxicity 8 d after, while PQQ were used or not before. The metamorphosis of hippocampal neurons was observed under the microscope. Intracellular calcium levels was measured by confocal laser microscopy. ResultsThe intracellular Ca2+ level increased rapidly after exposure to 0.1 mmol/L NMDA and resulted in neuronal necrosis and apoptosis. Otherwise, pretreatment of the cultured neurons with PQQ reduced the increase of the intracellular Ca2+ level and the neuronal necrosis or apoptosis induced by NMDA.ConclusionPQQ can protect hippocampal neurons from damage induced by NMDA.

Key words: pyrroloquinoline quinone (PQQ), hippocampal neurons, N-methyl-D-aspartate(NMDA), rat