《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (06): 441-442.

• 基础研究 • 上一篇    下一篇

中药膜贴预处理对随后佐剂性关节炎大鼠早期炎症的影响

周登芳; 李晓泓; 张露芬; 倪健; 翟景慧; 李辉; 李敬道   

  1. 1.北京中医药大学针灸学院 北京市 100029;2.北京中医药大学中药学院 北京市 100029
  • 收稿日期:2005-01-18 出版日期:2005-06-25 发布日期:2005-06-25

Effect of dressing-pretreatment of Chinese Traditional Medicine membranous plaster on forepart inflammation in adjuvant arthritis rats

ZHOU Deng-fang, LI Xiao-hong, ZHANG Lu-fen, et al   

  1. School of Acupuncture and Moxibustion of Beijing University of TCM, Beijing 100029, China
  • Received:2005-01-18 Published:2005-06-25 Online:2005-06-25

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摘要： 目的探讨贴敷预处理对随后佐剂性关节炎(AA)大鼠炎症的影响。方法应用福氏完全佐剂制作AA动物模型,24只Wistar大鼠随机分为正常组、AA组和预贴敷组(自制中药膜剂贴敷大鼠大椎穴),观察3组大鼠关节局部炎症和血清IL-1、IL-6、TNF-α含量。结果预贴敷组、AA组大鼠的足肿胀率和炎性因子与正常组有显著性差异;与AA组相比,预贴敷组大鼠足肿胀程度明显减轻(P<0.01),TNF-α含量有下降趋势,IL-1和IL-6含量下降(P<0.01和P<0.05),而且HE染色关节滑膜及软骨炎细胞浸润、血管增生及软骨破坏亦明显减轻。结论贴敷预处理能减轻随后AA大鼠早期炎症反应和关节病理损害程度,具有一定抵抗随后疾病损伤的保护作用。

关键词: 预处理, 中药贴敷, 佐剂性关节炎, 大椎穴

Abstract: ObjectiveTo explore the effect of dressing-pretreatment on forepart inflammation in adjuvant arthritis (AA) rats.MethodsAA model was established by subcutaneous injection of Freund's complete adjuvant into the right hind-paw (0.1 ml/animal). 24 Wistar rats were randomly divided into the control group, model group and dressing-pretreatment groups. In dressing-pretreatment group, "Dazhui" (GV14) was applied by a kind of dressing invented by the researchers. The foot swelling rate, serum concentrations of interleukin-1 (IL-1), IL-6 and tumor necrotic factor-α (TNF-α) were assessed.ResultsCompared with the control group, the foot swelling rate and serum concentrations of IL-1, IL-6 and TNF-α of the model group and dressing-pretreatment group increased significantly (P<0.01); but the foot swelling rate and serum concentrations of IL-1 and IL-6 in dressing-pretreatment group decreased significantly compared with those of the model group (P<0.05～0.01), and there was a downtrend in TNF-α. HE stain showed soakage of phlogistic cell in cartilage and arthritis membrane, hyperplasia of vein and breakage of cartilage were better in dressing-pretreatment group compared with that in the model group.ConclusionDressing-pretreatment can decrease forepart inflammation and pathological damage of arthritis in AA rats, and can prevent the harm of consequent disease to some extent.

Key words: pretreatment, Chinese Traditional Medicine dressing, adjuvant arthritis, Dazhui point