《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (06): 419-421.

• 专题 • 上一篇    下一篇

自体骨髓单核细胞移植治疗急性心肌梗死实验研究

姜雪松1; 于玲范1; 刘文华1; 王晓旭3; 秦燕2; 李姝娜2; 吕勃1   

  1. 1.哈尔滨医科大学附属第二临床医学院心外监护中心 黑龙江哈尔滨市 150086;2.哈尔滨医科大学附属第二临床医学院心脏彩超室 黑龙江哈尔滨市 150086;3.威海市立二院心内科 山东威海市 264200
  • 收稿日期:2004-08-15 出版日期:2005-06-25 发布日期:2005-06-25

Autologous bone-marrow mononuclear cells transplantation in experimental acute myocardial infarction

JIANG Xue-song, YU Ling-fan, LIU Wen-hua, et al   

  1. The Department of ICU, The Second Affiliated Clinical Hospital of Harbin Medical University,Harbin 150086, Heilongjiang, China
  • Received:2004-08-15 Published:2005-06-25 Online:2005-06-25

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摘要： 目的探讨自体骨髓单核细胞(ABM-MNCs)移植至心肌梗死区及周边区后的存活、分化状况,以及对心功能的影响。方法将40只日本大耳雄兔随机分为细胞移植组和对照组各20只。采用结扎左冠状动脉前降支的方法建立急性心肌梗死(AMI)模型。细胞移植组于梗死后第6天于自体髂骨处抽取骨髓,分离得到单核细胞后,以Brdu标记,经心外膜注射到梗死区及周边区。对照组仅注射等量的生理盐水。移植6周后,进行组织学及免疫组织化学检查。结果移植后6周,两组动物的心功能均有改善,但两组间有显著性差异。Brdu标记示移植组梗死区内存在阳性染色的移植细胞,而周边区可见Brdu染色阳性的心肌细胞及血管内皮细胞。免疫荧光染色示梗死区内Brdu染色阳性的移植细胞抗心肌特异性肌动蛋白抗体阴性,而周边区内Brdu染色阳性的移植细胞抗心肌特异性肌动蛋白抗体阳性。对照组未发现Brdu染色阳性的细胞。移植组梗死周边区及远离梗死区的毛血管密度均高于对照组(P<0.05),但梗死区内的毛细血管密度两组间无显著性差异(P>0.05)。结论ABM-MNCs移植至急性梗死心肌后,可在梗死区内及周边区存活,并可在周边区分化为具有心肌功能的细胞及血管内皮细胞,增加梗死周边区的血管密度,改善心功能。

关键词: 自体, 骨髓单核细胞, 细胞移植, 心肌梗死

Abstract: ObjectiveTo investigate the survival and differentiation of autologous bone-marrow mononuclear cell (ABM-MNCs) after transplanted to infarcted area and border area, and the effect of ABM-MNC on the cardiac function.Methods40 male big-ear Japanese rabbits were divided randomly into the transplanted group and control group with 20 animals in each group. Acute myocardial infarction model was made by ligating left anterior descending artery. 7 days later, Brdu labeled ABM-MNCs were injected into myocardium in the transplanted group, while the control rabbits were injected with saline. Six weeks later, tests of histology and immunohistochemistry were performed.ResultsViable cells labeled with Brdu can be identified in the infarcted area, and myocytes and endothelial cells labeled with Brdu can also be found in the border area, these cells demonstrated myogenic differentiation with the expression of α-actin by immunostaining. While, no cells labeled with Brdu were found in the control group. Moreover, the vessel density of the transplanted group in the borders of the infarction was higher than the control group (P<0.05), but there was no difference in infarcted area between two groups (P>0.05).At the 6 weeks after experiment, the cardiac function was improved in both groups, but there was a significant difference between two groups (P<0.05).ConclusionABM-MNCs injected into the infarcted myocardium can survive in both the infarcted and border areas, and differentiate into endothelial cells and other cells which are able to obtain the characters of myocytes, and increase the vessel density in border area, improve the cardiac function.

Key words: autologous, bone-marrow mononuclear cells, cell transplantation, myocardial infarction