《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (05): 343-344.

• 基础研究 • 上一篇    下一篇

Caspase-3在戊四氮急性致痫大鼠脑组织中的表达

郭小明; 王薇薇; 王荫华; 吴逊   

  1. 北京大学第一医院神经内科 北京市 100034
  • 收稿日期:2005-01-13 出版日期:2005-05-25 发布日期:2005-05-25

Expression of caspase-3 in the rat brain following pentylenetetrazole-induced epilepsy

GUO Xiao-ming, WANG Wei-wei, WANG Yin-hua, et al   

  1. Department of Neurology, Peking University First Hospital,Beijing 100034, China
  • Received:2005-01-13 Published:2005-05-25 Online:2005-05-25

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摘要： 目的研究casPase-3在戊四氮(Pentylenetetrazole,PTZ)急性致痫大鼠脑组织中的表达情况。方法免疫组织化学SP法显示海马及皮层casPase-3阳性细胞,CMIAS图像分析系统半定量分析casPase-3蛋白的含量。结果癫痫发作后海马及皮层casPase-3蛋白的表达水平均增加,且海马组织较皮层组织增加更为明显。结论CasPase-3 参与了癫痫后脑组织神经元的凋亡过程;在癫痫发作所致的神经元损伤中,海马较皮层可能更为敏感。

关键词: 癫痫, Caspase-3, 细胞凋亡, 戊四氮, 大鼠

Abstract: ObjectiveTo investigate the expression of caspase-3 in the brain of acutely pentylenetetrazole(PTZ)-kindled rats.MethodsThe caspase-3 positive cells were revealed using immunohistochemical SP method. CMIAS image analysis system was used to analyse the expression of caspase-3 hemi-quantitatively. ResultsFollowing PTZ induced epilepsy, the expression of caspase-3 increased both in the hippocampus and in the cortex, and that was more remarkable in the hippocampus than in the cortex.ConclusionCaspase-3 may be activated during neuronal apoptosis after epilepsy. Hippocampus is more sensitive to the neuronal damage due to epilepsy than the cortex is.

Key words: epilepsy, caspase-3, cell apoptosis, pentylenetetrazole, rat