《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (05): 339-340.

• 基础研究 • 上一篇    下一篇

血清预培养促进神经干细胞的增殖

历俊华; 郑淑荣; 万虹; 张绍东   

  1. 北京市神经外科研究所 北京市 100050
  • 收稿日期:2005-01-31 出版日期:2005-05-25 发布日期:2005-05-25

Pro-culture with serum promoting neural stem cell proliferation

LI Jun-hua, ZHENG Shu-rong, WAN Hong, et al   

  1. Beijing Neurosurgical Institute, Beijing 100050, China
  • Received:2005-01-31 Published:2005-05-25 Online:2005-05-25

摘要: 目的介绍一种时间短、花费少的神经干细胞培养方法。方法先用含10%胎牛血清的DMEM培养液预培养神经干细胞48h,换含表皮生长因子、碱性成纤维细胞生长因子、B27的DMEM/F12培养液继续培养干细胞球。血清诱导分化后行nestin免疫荧光染色。结果血清预培养48h后即可见神经干细胞聚球,nestin免疫荧光染色为阳性。结论血清预培养可使神经干细胞聚球速度加快,促进神经干细胞增殖。

关键词: 神经干细胞, 预培养, 血清, 增殖

Abstract: ObjectiveTo introduce a method of promoting neural stem cells proliferation.MethodsNeural stem cells were cultured with medium DMEM containing 10% fatal bovine serum for 48 h, then changed medium DMEM/F12 containing epidermal growth factor, basic fibroblast growth factor and B27. Immunohistochemistry was used to identify nestin expression.ResultsNeural stem cells got together fast and formed neurospheres within 48 h and expressed nestin. ConclusionPro-culture with serum can promotes neural stem cell proliferation.

Key words: neural stem cell, pre-culture, serum, proliferation