《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (05): 327-328.

• 专题 • 上一篇    下一篇

β-淀粉样肽损伤后小鼠脑单胺氧化酶B、白细胞介素6的变化及中药的干预作用

张孟仁; 郭赛珊; 左萍萍; 杨楠   

  1. 北京协和医院中医科; 中国协和医科大学基础医学院药理系
  • 收稿日期:2005-02-17 出版日期:2005-05-25 发布日期:2005-05-25

Effects of β-amyloid peptide on the monoamine oxidase-B, interleukin-6 in the brain of mice and the interfering effect of traditional Chinese medicine

ZHANG Men-ren, GUO Sai-shan,ZUO Ping-ping, et al   

  1. Peking Union Medical College Hospital, Institute of Basic Medical Sciences, PUMC & ACMS,100730 Beijing,China
  • Received:2005-02-17 Published:2005-05-25 Online:2005-05-25

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摘要： 目的观察β-淀粉样肽(Aβ)25-35致痴呆小鼠模型脑单胺氧化酶B(MAO-B)、白细胞介素6(IL-6)的改变及中药脑复聪的干预作用。方法脑室内注射Aβ25-35造成痴呆模型小鼠,用Morris水迷宫测空间学习记忆功能;用比色法测MAO-B活性;免疫组化检测IL-6。结果模型小鼠的Morris水迷宫潜伏期(81.3±13.4)s,比对照组(34.2±10.9)s延长(P<0.05);大脑皮层和海马的MAO-B活性(120.12±10.15)、(83.60±5.29)均较对照组(93.09±10.54)、(50.39±9.16)升高(P<0.05～0.01),海马齿状回有大量IL-6免疫荧光阳性细胞。经脑复聪治疗后,水迷宫潜伏期(43.7±12.7)s缩短(P<0.05);海马的MAO-B活性(47.11±6.57)回降(P<0.01);IL-6免疫阳性神经细胞减少。结论抑制海马MAO-B的过度激活,减少自由基的产生,减少氧化应激反应,抑制小胶质细胞产生炎性因子IL-6,缓解Aβ的损伤作用,阻断AD发病的重要环节,是中药脑复聪治疗AD的重要机理之一。

关键词: β-淀粉样肽, 痴呆, 单胺氧化酶B(MAO-B), 白细胞介素-6(IL-6), 脑复聪, 小鼠

Abstract: ObjectiveTo observe the changes of monoamine oxidase-B (MAO-B), interleukin-6(IL-6) in the cortex and hippocampus of Alzheimer's disease model mice and the effect of traditional Chinese medicine Naofucong.MethodsAlzheimer's disease model was induced in mice by β-amyloid(Aβ)25-35 icv. Space learning and memorial ability was tested in Morris water maze. The activity of MAO-B was measured by colorimetric method. IL-6 was observed with the immnuohistochemical stain.ResultsMice in the model group presented longer latent periods of Morris water maze(81.3±13.4)s, higher activities of MAO-B in brain cortex and hippocampus (120.12±10.15,83.60±5.29) compare with that of the control group, which was (34.2±10.9)s,(93.09±10.54) and (50.39±9.16)(P<0.05～0.01).There were many IL-6 positive cell in dentate gyrus of hippocampus of the model group. After administration with Naofucong grain, latent periods (43.7±12.7) s and activities of MAO-B (47.11 ± 6.57)in hippocampus were recovered(P<0.05～0.01), and the IL-6 positive cell in dentate gyrus decreased.ConclusionNaofucong grain can antagonize the Aβ25-35 toxicity by decreasing the overactivition of MAO-B and the excretion of inflammatory medium by microglia, as well as improve the memory function.

Key words: β-amyloid peptide, dementia, monoamine oxidase-B (MAO-B), interleukin-6(IL-6), Naofucong, mice