《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (09): 806-807.

• 专题 • 上一篇    下一篇

参龙健脑汤对D-半乳糖诱导衰老大鼠脑神经递质的影响

陈利平1a; 王发渭1a; 韩志涛1b; 臧倩1a   

  1. 1.中国人民解放军总医院a.中医科;b.老年病研究所,北京市 100853
  • 收稿日期:2008-05-09 出版日期:2008-09-01 发布日期:2008-09-01

Effect of Shenlongjiannaotang on Neurotransmitters in D-Glalactose Induced Aging Mimetic Rats

CHEN Li-ping, WANG Fa-wei,HAN Zhi-tao, et al   

  1. Department of Chinese Traditional Medicine, the General Hospital of PLA, Beijing 100853, China
  • Received:2008-05-09 Published:2008-09-01 Online:2008-09-01

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摘要： 目的探讨参龙健脑汤对D-半乳糖拟衰老大鼠学习记忆能力的影响,以及对拟衰老大鼠大脑皮质内去甲肾上腺素、多巴胺和5-羟色胺浓度变化的影响。方法采用D-半乳糖诱导的脑老化大鼠为动物模型,将实验动物分为5组:正常对照组、参龙健脑汤低剂量组、中剂量组、高剂量组、D-半乳糖诱导模型组,采用不同剂量的参龙健脑汤灌胃,分别用游泳水迷宫测定各组大鼠学习记忆能力的变化;采用荧光法测定各组大鼠大脑皮质内去甲肾上腺素、多巴胺和5-羟色胺浓度的变化。结果与正常对照组相比,D-半乳糖模型组大鼠游泳时间延长,游泳正确次数降低(P<0.05)。与模型组比较,应用大剂量参龙健脑汤处理6周后,大鼠游泳时间降低,正确次数增加(P<0.05);大鼠脑内去甲肾上腺素、5-羟色胺的浓度升高(P<0.05)。结论参龙健脑汤能改善D-半乳糖诱导的脑老化大鼠学习记忆能力,并能提高脑组织中去甲肾上腺素、5-羟色胺浓度。上述作用呈剂量相关。

关键词: 衰老, 参龙健脑汤, 半乳糖, 中药, 记忆, 去甲肾上腺素, 5-羟色胺, 大鼠

Abstract: Objective To investigate the influence of Shenlongjiannaotang on the function of learning and memory of D-glalactose induced aging mimetic rats,and the concentrations of norepinephrine(NE),dopamine(DA) and 5-hydroxy tryptamine(5-HT) in cerebrum.Methods 50 rats were randomly divided into 5 groups: normal control group,model group,and high/middle/low dosage prescription group of Shenlongjiannaotang.The memory function was tested with Morris water maze.The concentrations of NE,DA and 5-HT were detected with HPLC with fluorescence spetrometer.Results In the model group,the latency of model mice increased,and the correct number decreased significantly compared with normal group(P<0.05).After 6 weeks administering Shenlongjiannaotang,the latency decreased and the correct number increased significantly in high dosage group,compared with those of the model group(P<0.05).The contents of NE and DA reduced in model group,and Shenlongjiannaotang could increase NE and DA contents of rats cerebrum(P<0.05).Conclusion Shenlongjiannaotang could improve the learning and memory function of D-galactose induced aging rats,which may related with the increase of NE and DA contents in cerebral tissue.The effects depend on the dosage of Shenlongjiannaotang.

Key words: senescence, Shenlongjiannaotang, D-glalactose, Chinese medicine, memory, norepinephrine, 5-hydroxy tryptamine, rats