《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (08): 731-732.

• 基础研究 • 上一篇    下一篇

骨髓基质细胞移植对大鼠局灶性脑损伤VEGF表达的影响

张鹏飞1,2; 张亚卓1,2; 李庆国1,2; 孙梅珍1,2; 王红云1,2; 何乐1,2   

  1. 1.首都医科大学附属北京天坛医院,北京市 100050;2.北京市神经外科研究所,北京市 100050
  • 收稿日期:2008-05-12 出版日期:2008-08-01 发布日期:2008-08-01

Effects of Transplantation of Bone Marrow Stromal Cells on Expression of Vascular Endothelial Growth Factor after Local Brain Injury in Rats

ZHANG Peng-fei, ZHANG Ya-zhuo, LI Qing-guo, et al   

  1. Beijing Tiantan Hospital Affiliated to Capital Medical University, Beijing Neurosurgical Institute, Beijing 100050, China
  • Received:2008-05-12 Published:2008-08-01 Online:2008-08-01

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摘要： 目的观察骨髓基质细胞(BMSCs)移植对局灶性脑损伤大鼠损伤局部脑组织血管内皮细胞生长因子(VEGF)表达的影响。方法制备大鼠局灶性脑损伤动物模型,进行BMSCs移植,通过免疫组织化学、荧光定量PCR、ELISA等方法观察移植局部脑组织VEGF以及VEGF mRNA的变化。结果BMSCs移植后,局部VEGF染色阳性细胞数增加;VEGF mRNA含量增高。结论BMSCs移植可促进内源性VEGF的表达,提高损伤局部VEGF的含量。

关键词: 骨髓基质细胞, 脑损伤, 血管内皮细胞生长因子

Abstract: Objective To observe the effects of transplantation of bone marrow stromal cells(BMSCs)on expression of vascular endothelial growth factor(VEGF)after local brain injury in rats.Methods The animal model of local brain injury in rat was established.The human BMSCs(hBMSCs)were transplanted into the local brain.The immunohistochemistry,real-time quantitative PCR,and ELISA were used to observe the changes of VEGF and VEGF mRNA in tissues of local brain.Results After the hBMSCs were transplanted into the animal model,the quantities of the VEGF positive cells increased;real-time quantitative PCR showed that VEGF mRNA of local brain was enhanced.Conclusion The BMSCs transplantation can improve expression of endogenous VEGF and raise the content of VEGF in injured tissues of brain.

Key words: bone marrow stromal cells, brain injury, vascular endothelial growth factor(VEGF)