《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (02): 125-126.

• 基础研究 • 上一篇    下一篇

Earle's液与MEM培养基对神经元活性的影响

韩明; 董丽萍; 袁芳   

  1. 北京市神经外科研究所 北京市 100050
  • 收稿日期:2004-07-15 出版日期:2005-02-25 发布日期:2005-02-25

Effect of Earle's solution and MEM on activities of neurons in culture

HAN Ming, DONG Li-ping, YUAN Fang   

  1. Beijing Neurosurgical Institute, Beijng 100050, China
  • Received:2004-07-15 Published:2005-02-25 Online:2005-02-25

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摘要： 目的 观察Earle's液、新配最小必需培养基(MEM)及原 MEM对神经元活性的影响。方法 神经元培养10d后,分别换为Earle's液、新配MEM及原MEM,12h和24h后用乳酸脱氢酶(LDH)试剂盒测定细胞的LDH漏出量,四甲基偶氮唑盐(MTT)微量酶反应比色法测定MTT代谢率。结果 神经元在不同培养介质中孵育 12 h和 24 h后,细胞LDH的漏出量及MTT代谢率有非常显著性差异(P<0.01)。结论 不同孵育液对神经元的活性有影响。

关键词: 神经元, Earle's液, 最小必需培养基(MEM), 乳酸脱氢酶, 四甲基偶氮唑盐(MTT)

Abstract: ObjectiveTo observe the activities of cultured neurons incubated with Earle's solution, new minimal essential medium (MEM) or former MEM.MethodsActivity changes of cultured neurons were measured by determining the leakage of lactate dehydrogenase (LDH) and metabolic rate of 3-(4,5-dimethylthiazol)2,5 diphenyltetrazolium bromide (MTT).ResultsActivities of neurons incubated with different culture medium for 12 h or 24 h were significantly different (P<0.01).ConclusionDifferent culture medium can influence neuronal activities.

Key words: neurons, Earle's solution, minimal essential medium (MEM), lactate dehydrogenase (LDH), 3-(4, 5-dimethylthiazol)2, 5-diphenyltetrazolium bromide (MTT)