《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (02): 94-95.

• 专题 • 上一篇    下一篇

针刺对慢性脊髓损伤大鼠神经递质和营养因子表达的影响

王新家; 孔抗美; 齐伟力   

  1. 汕头大学医学院第二附属医院脊柱外科 广东汕头市 515041
  • 收稿日期:2004-11-12 出版日期:2005-02-25 发布日期:2005-02-25

Effect of acupuncture on the expression of nerve transmitter and neurotrophic factor in rats with chronic spinal cord injury

WANG Xin-jia, KONG Kang-mei, QI Wei-li   

  1. Department of Spine Surgery, The Second Affiliated Hospital, Shantou University Medical College, Shantou 515041, Guangdong, China
  • Received:2004-11-12 Published:2005-02-25 Online:2005-02-25

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被引次数

51

摘要： 目的 探讨针刺治疗慢性脊髓损伤的机理。方法 建立后路渐进性脊髓压迫大鼠模型,然后手术减压,进行电针治疗,观察联合行为评分(CBS)及胆碱乙酰转移酶(ChAT)、脑源性神经营养因子(BDNF)及其受体TrkB的免疫组化检测结果。结果 初次术后90 d,电针组大鼠CBS评分优于减压组(P<0.05),ChAT免疫组化染色阳性细胞数多于减压组 (P<0.05),神经元和胶质细胞增强的BDNF和TrkB表达得到恢复。结论 电针治疗可能通过影响 BDNF及其受体的表达,促进 ChAT表达和增强其活性而对大鼠慢性脊髓损伤起治疗作用。

关键词: 电针, 脊髓损伤, 脑源性神经营养因子, 胆碱乙酰转移酶

Abstract: ObjectiveTo explore the mechanism of electro-acupuncture (EA) on chronic spinal cord injury.MethodsAn animal model of moderate chronic progressive compression on spinal cord was made. Then the animals were decompressed and treated with EA. The combined behavioral scores (CBS) were tested, and the expressions of choline acetyltransferase (ChAT), brain derived neurotrophic factor (BDNF) and its receptor TrkB on the section of the rat's spinal cord were examined with immunohistochemical method.Results90 days after injury, the CBS of EA group was significantly better than that of decompression group ( P<0.05) and the number of positive ChAT cells in ventral horn of spinal cord in EA group was more than that in decompression group (P<0.05). The expressions of BDNF and TrkB enhanced after injury were recovered in the EA group.ConclusionThe electro-acupuncture may be effect on chronic spinal cord injury in rats by influencing the expressions of BDNF and its receptor, and improvs the expression and activity of ChAT.

Key words: electro acupuncture (EA), spinal cord injury (SCI), brain derived neurotrophic factor (BDNF), choline acetyltransferase (ChAT)