《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (03): 209-211.

• 专题 • 上一篇    下一篇

通心络对脑梗死大鼠的脑缺血耐受及Bcl-2、Bax蛋白表达的影响

罗方1a; 吴锦晖1a; 罗祖明1b   

  1. 1.四川大学华西医院,a.老年科;b.神经内科,四川成都市 610041
  • 收稿日期:2007-12-18 出版日期:2008-03-01 发布日期:2008-03-01

Effects of Tongxinluo on Cerebral Ischemic Tolerance and Expression of Bcl-2 and Bax in Rats with Cerebral Infarction

LUO Fang,WU Jin-hui, LUO Zu-ming   

  1. Department of Geriatrics, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China
  • Received:2007-12-18 Published:2008-03-01 Online:2008-03-01

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摘要： 目的探讨Bcl-2、Bax蛋白对脑缺血耐受的作用及通心络的干预作用。方法检测不同时程通心络组、全脑缺血-局灶脑缺血再灌注组及单纯局灶脑缺血组大鼠的神经行为评分、脑梗死体积,免疫组化法检测Bcl-2、Bax蛋白阳性细胞数,TUNEL法检测神经细胞的凋亡。结果通心络组的神经行为评分降低,脑梗死体积减少,脑缺血半暗带的Bcl-2蛋白表达增高而Bax蛋白表达减少,神经细胞的凋亡减少。结论通心络增加脑缺血半暗带Bcl-2蛋白的表达,抑制Bax蛋白的表达,从而抑制神经细胞凋亡,增强缺血预处理诱导的脑缺血耐受。

关键词: 通心络, 缺血耐受, Bcl-2, Bax, 凋亡, 大鼠

Abstract: Objective To observe the effects of Tongxinluo on cerebral ischemic tolerance and expression of Bcl-2 and Bax in rats with cerebral infarction.Methods The neurophysical assessment and the volume of the cerebral infarction in Tongxinluo group,middle cerebral artery occlusion(MCAO)group,preconditioning and MCAO(PC-MCAO)group were determined at different time point.The expression of Bcl-2 and Bax were measured with immunohistochemitry and the apoptosis of the nerve cells were detected with TUNEL.Results Among these groups in the corresponding time,the neurophysical marks of Tongxinluo group was the lowest and the cerebral infarction volume was the smallest at 24 h,3 d and 5 d points.The Bcl-2 positive neurons were the highest,while the Bax positive neurons and the apoptosis rate of nerve cells were the lowest around the necrotic areas in Tongxinluo group(P<0.05).Conclusion Tongxinluo can improve the cerebral ischemic tolerance and increase the expression of Bcl-2 and decrease that of Bax around the necrotic area after cerebral ischemia in rats.

Key words: Tongxinluo, ischemic tolerance, Bcl-2, Bax, apoptosis, rats