《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (03): 206-208.

• 专题 • 上一篇    下一篇

实验性脑出血血肿周围组织细胞凋亡的研究

李红玲1a; 葛艳萍1a; 郭艳苏1b; 吴东霞1b; 李春岩1b   

  1. 1.河北医科大学第二医院,a.康复科;b.神经内科;河北石家庄市 050000
  • 收稿日期:2007-12-13 出版日期:2008-03-01 发布日期:2008-03-01

Apoptosis of Neuron Surrounding Intracerebral Hemorrhage in Rats

LI Hong-ling, GE Yan-ping, GUO Yan-su, et al.   

  1. Department of Rehabilitation, The Second Hospital of Hebei Medical University, Shijiazhuang050000, Hebei, China
  • Received:2007-12-13 Published:2008-03-01 Online:2008-03-01

PDF (PC)

被引次数

21

摘要： 目的探讨脑出血大鼠血肿周围组织细胞凋亡的发生、发展情况。方法64只SD大鼠被随机分为2组,实验组(56只)采用胶原酶诱导大脑尾状核脑出血模型,对照组(8只)为假手术组,实验组分为术后6h、12h、24h、48h、72h、7d、14d7个时相点,运用TUNEL、SP技术和电子显微镜检测血肿周围组织中细胞凋亡及caspase-3蛋白表达。结果TUNEL阳性细胞数在实验组6h时可见,24～48h表达明显,与对照组相比有显著性差异(P<0.05)。caspase-3蛋白表达规律与TUNEL一致,但高峰时间早于TUNEL。透射电镜可见细胞凋亡形态学改变。结论脑出血后血肿周围组织存在神经细胞凋亡,且与caspase-3蛋白表达有关。

关键词: 脑出血, 凋亡, caspase-3, 大鼠

Abstract: Objective To investigate the apoptosis of neuron surrounding the hematoma in intracerebral hemorrhage(ICH)rats.Methods 64 male SD rats were randomly divided into two groups,trial group(ICH,n=56)and control group(sham operated,n=8).The brains of the rats were removed 6 h,12 h,24 h,48 h,72 h,7 d,14 d after ICH.Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-bioti in situ nick end-labeling(TUNEL)was used to detect deoxyribonucleic acid(DNA)fragmentation.The activation of caspase-3 was measured with immunohistochemistery.The electron microscope were used to observe histological changes surrounding the hematoma.Results Under transmission electronic microscope,shrunken neuron and glial cell with pre-apoptotic signs of intensely stained cytoplasm and abnormally dense nucleus,swollen blood vessel were found.TUNEL-positive cells appeared in the periphery of the hematoma and increased from 6 h to 14 d after ICH.Little TUNEL-positive cells could be found in the control group.The change of the caspase-3-positive cells was similar to TUNEL,but the peak of caspase-3-positive cells was more early than that of TUNEL.Conclusion The apoptosis of neuron occurred surrounding the hematoma in ICH rats and it may related to caspase-3.

Key words: intracerebral hemorrhage(ICH), apoptosis, caspase-3, rats