《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (02): 84-86.

• 专题 • 上一篇    下一篇

重组人红细胞生成素对大鼠脊髓损伤后神经细胞凋亡的保护作用

王金光; 郑启新; 王运涛; 赵铭; 郭晓东   

  1. 华中科技大学同济医学院附属协和医院骨外科 湖北武汉市 43000002
  • 收稿日期:2004-11-12 出版日期:2005-02-25 发布日期:2005-02-25

Protective effect of recombinant-Human Erythropoietin on neuronal apoptosis after spinal cord injury in rats

WANG Jin-guang,ZHENG Qi-xin,WANG Yun-tao, et al   

  1. Department of Orthopedics,Union Hospital,Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022,Hubei, China
  • Received:2004-11-12 Published:2005-02-25 Online:2005-02-25

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摘要： 目的 观察大鼠脊髓损伤(SCI)后损伤段脊髓神经细胞凋亡机制和相关因子的表达,以及重组人红细胞生成素(rHu-EPO)对其的影响。方法 30只SD大鼠分为4组,建立SCI模型,观察rHu EPO对大鼠神经功能的影响;免疫组化法检测损伤脊髓神经细胞凋亡因子(Bcl 2、Bax、Fas)的表达;TUNEL标记凋亡细胞;比较各组间差别。结果 治疗组的神经功能较损伤组提高(P<0.05);损伤组的凋亡阳性细胞多于治疗组;治疗组Bcl 2阳性表达细胞多于损伤组(P<0.05)。结论 凋亡是 SCI后神经细胞死亡的一种重要方式;rHu-EPO能抑制脊髓神经细胞凋亡,对损伤脊髓的神经功能有保护作用。

关键词: 脊髓损伤, 凋亡, 重组人红细胞生成素, β-七叶皂甙钠, 大鼠

Abstract: ObjectiveTo observe the expression of apoptosis factor, the mechanism of neuronal apoptosis and profective effect of recombinant Human Erythropoietin (rHu EPO) after spinal cord injury (SCI) in SD rats.Methods30 SD rats were divided into four groups including control group, SCI group, treatment groups A and B.The animal SCI model was established with Allen's method. The changes of nerve functions of rats of 4 groups were observed before and after treating with rHu EPO. The expressions of apoptosis factors (Bcl-2,Bax,Fas) were tested with immunocytochemistry technique, and apoptosis neurones were labeled with TUNEL dyeing.ResultsThe grade of nerve function was improved distinctly in treatment groups, but group B was better than group A.The number of the positive cells for Fas and Bax in SCI group was more than that in treatment groups (P<0.05), and group A was more than group B. The number of Bcl 2 positive cells in the treatment groups was greater than that in SCI group (P<0.05).ConclusionApoptosis is a important death mode of neuron after SCI. rHu EPO can distinctly improve the comeback of the nerve function and protect the nerve tissue.

Key words: spinal cord injury, apoptosis, recombinant Human Erythropoietin (rHu EPO), sodium β aescin, rats