《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (10): 590-591.

• 基础研究 • 上一篇    下一篇

大鼠延髓呼吸性神经元向脊髓膈运动神经元投射的实验观察

王利清1; 景德强2; 张素贞2   

  1. 1.北京博爱医院外科 北京市 100068;2.第三军医大学解剖教研室 重庆市 400038
  • 收稿日期:2004-08-15 出版日期:2004-10-25 发布日期:2004-10-25

Projections from respiratory neurons of medulla oblongata to the motor neuron pool of phrenic nerve in rat:An HRP retrograde tracing study

WANG Li-qing, JING De-qiang, ZHANG Su-zhen   

  1. Beijing Charity Hospital, Beijing 100068, China
  • Received:2004-08-15 Published:2004-10-25 Online:2004-10-25

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被引次数

5

摘要： 目的观察大鼠延髓呼吸性神经元向脊髓膈运动神经元的直接投射。方法25只Wistar大鼠分为3组:脊髓定位组(5只)、延髓投射组(15只)和对照组(5只)。运用辣根过氧化物酶(HRP)逆追踪法,于脊髓定位组膈神经干注入HRP,确定膈运动神经元在脊髓中的位置,然后延髓投射组于逆行性标记细胞位置,对照组于脊髓后角分别注入HRP,观察延髓呼吸性神经元的标记情况。结果在脊髓定位组的注射同侧观察到,C3—C5节段的前角中间部出现逆行性标记细胞,为典型的前角运动神经元。在延髓投射组观察到,逆行性标识神经元出现在延髓疑后核和面后核的腹内侧部。对照组在上述位置未见标识神经元。结论大鼠膈运动神经元位于C3-C5节段,其投射神经元主要分布在以延髓疑后核和面后核区为主轴的一个长的细胞柱上。

关键词: 辣根过氧化物酶, 大鼠, 膈运动神经元, 延髓, 呼吸性神经元, 疑后核, 面后核

Abstract: ObjectiveTo observe the projections from respiratory neurons of medulla oblongata to the motor neuron pool of phrenic nerve in rat. MethodsBy using horseradish peroxidase (HRP) retrograde tracing technique after injecting HRP into phrenic nerve, retrograde labelled neurons were found in C3-C5 segment. Then, HRP was injected into the area where the phrenic motor neurons occupied, retrograde labelled neurons were found in medulla oblongata. ResultsPhrenic motor neurons locate in the C3-C5 segment ipsilaterally, occuping the intermediate portion of anterior horn and appearing typical motor neurons. Retrograde labelled neurons were found bilaterally in medulla oblongata, the neurons were located in nucleus retroambigualis (RNA), ventramedial area to nucleus retrofacialis (NRF). ConclusionThe phrenic motor neurons may receive direct projection of respiratory neuron in medulla oblongata, the projecting neurons are distributed in RNA and NRF area.

Key words: horseradish peroxidase (HRP), rat, phrenic motor neuron, medulla oblongata, respiratory neuron, nucleus retroambigualis (RNA), nucleus retrofacialis (NRF)