《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (10): 585-587.

• 基础研究 • 上一篇    下一篇

有氧运动对胰岛素抵抗大鼠脂肪组织蛋白激酶B表达的影响

李建辉1; 毕会民1; 甘佩珍1; 汪保华2   

  1. 1.武汉大学人民医院内分泌科 湖北武汉市 430060;2.荆门市第一人民医院神经内科 湖北荆门市 448000
  • 收稿日期:2004-08-15 出版日期:2004-10-25 发布日期:2004-10-25

Effect of exercise on expression of protein kinase B in adipose tissue of rats

LI Jian-hui, BI Hui-min, GAN Pei-zhen, et al   

  1. Department of Endocrinology, the People’s Hospital of Wuhan University, Wuhan 430060, Hubei, China
  • Received:2004-08-15 Published:2004-10-25 Online:2004-10-25

PDF (PC)

摘要： 目的在高脂饮食诱导胰岛素抵抗的基础上,观察有氧运动对胰岛素抵抗大鼠脂肪组织中蛋白激酶B(PKB)表达的影响。方法选取雄性Wistar大鼠30只,随机分为正常对照组10只,给予基础饲料;模型组20只,给予高脂饲料喂养4周后,再随机分为胰岛素抵抗组(继续高脂饮食)和运动处理组(高脂饮食+游泳运动)。干预6周后,采用蛋白印迹法检测大鼠脂肪组织中胰岛素刺激PKB表达的含量。结果在胰岛素抵抗状态时,大鼠脂肪组织PKB表达明显减少,较对照组下降23.5%(P<0.01);6周游泳运动后,运动组大鼠PKB表达明显增加,较胰岛素抵抗组增加19.2%(P<0.01)。结论运动能改善胰岛素抵抗,可能与增加脂肪组织中PKB表达有关。

关键词: 运动, 胰岛素抵抗, 脂肪组织, 蛋白激酶B

Abstract: ObjectiveTo observe the effect of exercise on expression of protein kinase B (PKB) in adipose tissue of insulin resistant rats fed by high fat diet.Methods30 male Wistar rats were randomly divided into the control group (n=10),given basic diet; the model group (n=20), given fat diet. After 4 weeks, the model group was randomly divided into 2 subgroups, the insulin resistant group was continually given high fat diet, the exercise treated group accepted high fat food and swimming training. After 6 week intervention, the expression of PKB stimulated by insulin in adipose tissue was determined with Western blotting at the end of experiment.ResultsAfter long term high fat diet, expression of PKB in adipose tissue of the insulin resistant group decreased by 23.5% comparing with the control group (P<0.01). After 6 weeks swimming training, the expression of PKB of the exercise treated group was increased by 19.2% comparing with the insulin resistant group (P<0.01).ConclusionExercise treatment can significantly elevate the expression of PKB and ameliorate the state of insulin resistance.

Key words: exercise, insulin resistance, adipose tissue, protein kinase B (PKB)