《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (05): 281-282.

• 基础研究 • 上一篇    下一篇

小分子化合物Salen-Mn的合成及其结构测定

郭金兰1; 张澄波2   

  1. 1.中国康复医学研究所 北京市 100068;2.首都医科大学生物化学教研室 北京市 100054
  • 收稿日期:2003-12-01 出版日期:2004-05-25 发布日期:2004-05-25

Synthesis and structure of the Salen-Mn compound

GUO Jin-lan, ZHANG Cheng-bo   

  1. Institution of Rehabilitation Medicine, Chinese Rehabilitation Research Centre, Beijing 100068, China
  • Received:2003-12-01 Published:2004-05-25 Online:2004-05-25

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摘要： 目的合成一种化合物,并对其结构及活性进行测定。方法采用回流方法,在实验室合成化合物,并通过质谱、红外光谱、核磁共振氢谱分析法对其结构进行检测,并用黄嘌呤氧化酶法测定此化合物的超氧化物岐化酶(SOD)酶活性。结果质谱分析确定此化合物分子量为356,红外光谱分析此化合物含有的官能团有-OH、C=N和苯环的存在,核磁共振氢谱分析证明有金属顺磁性物质锰(Mn)的存在,并具有一定的SOD酶活性。结论可合成一种低分子量的具有SOD酶活性的物质。

关键词: 超氧化物岐化酶, 自由基, 合成

Abstract: ObjectiveTo synthesize metal complex that exhibits superoxide dismutase (SOD)-like activity and determine its structure.MethodsThis chemical compound was synthesized by means of reflux in laboratory.Its structure was examined by infrared spectra (IR),nuclear magnetic resonance hydrogen spectra(H-NMR) and mass spectra (MS).The activity of the SOD was determine by xanthine oxidase (XOD).ResultsThe IR indicted that this complex showed a typical absorption band of -OH,C=N and benzene. The H-NMR reveals the existence of metal ion manganese (Mn3+ ,paramagnetic). The molecular weight was 365, which was obtained by EI-MS analysis. And this complex showed SOD-like activity.ConclusionA metal complex with low molecular weight and SOD-like activity has been synthesized.

Key words: superoxide dismutase(SOD), free radical, complex synthesis