《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (09): 534-535.

• 基础研究 • 上一篇    下一篇

白细胞介素10对实验性重症胰腺炎病理评分的影响研究

岳军忠1; 谷俊朝2; 薛建国2; 周延忠2   

  1. 1.北京博爱医院普通外科 北京市 100068;2.首都医科大学附属北京友谊医院普外科 北京市 100050
  • 收稿日期:2004-02-10 出版日期:2004-09-25 发布日期:2004-09-25

Effect of interleukin-10 on experimental severe acute pancreatitis's histopathologic score in rats

YUE Jun-zhong, GU Jun-zhao, XUE Jian-guo, et al   

  1. Department of Surgery, Beijing Charity Hospital, Beijing 100068, China
  • Received:2004-02-10 Published:2004-09-25 Online:2004-09-25

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被引次数

4

摘要： 目的探讨白细胞介素10(IL-10)对大鼠重症胰腺炎(SAP)胰腺病理及血清淀粉酶的影响。方法48只SD大鼠随机分为假手术组、SAP组和IL-10治疗组,用5%牛磺胆酸钠逆行胰胆管注射,制成SAP模型。结果SAP组和IL-10治疗组均有不同程度的胰腺间质水肿、腺泡坏死、炎性细胞浸润和间质出血。制模后6h和12h的胰腺病理评分IL-10治疗组(4.00±0.33和6.2 5±0.25)比SAP组(6.13±0.35和9. 50±0.50)明显降低(P<0.01);IL-10治疗组的血清淀粉酶(AMY)浓度在6h时,比SAP组低(P<0.05),在12h时,与SAP组无显著性差异(P>0.05)。结论早期应用IL-10能降低大鼠重症急性胰腺炎血清AMY的浓度,明显减轻胰腺间质水肿、腺泡坏死、炎性细胞浸润和间质出血等病理改变。

关键词: 白细胞介素10, 胰腺炎, 血淀粉酶, 病理评分

Abstract: ObjectiveTo explore effect of interleukin-10 (IL-10) on histopathologic scores of severe acute pancreatitis (SAP) and the level of serum amylase (AMY) in rats.Methods48 female and male adult Sprague Dawley rats (200—300g) were randomly allocated into three groups: OPgroup, SAP group and IL-10 group with 16 rats in each group. SAP was made with retrograde ductal infusion of 5% sodium taurocholate solution.ResultsIn SAP and IL-10 group, there were interstitial edema, necrosis, neutrophil infiltration and interstitial hemorrhage of pancreas, more or less. At 6h and 12h after models were made, the pancreatic histopathologic score in IL-10 group (4.00±0.33 and 6.25±0.25) were significantly lower than that in SAP group (6.13±0.35 and 9.50±0.50)(P<0.01). At 6 h after models were made, the serum AMY in IL-10 group was lower than that in SAP group (P<0.05), but at 12 h there were no differences.ConclusionIn earlier period of SAP in rats, IL-10 can lower the serum AMY level, and significantly reduced pancreatic histologic score (edema, inflammation, hemorrhage, and necrosis).

Key words: interleukin-10 (IL-10), pancreatitis, amylase (AMY), histopathologic score