《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (09): 532-533.

• 基础研究 • 上一篇    下一篇

双龙丸对兔主动脉粥样斑块内血管内皮生长因子和碱性成纤维细胞生长因子表达的影响

薛金贵1; 胡婉英1; 杨祖福2   

  1. 1.上海曙光医院心内科 上海市 200021;2.北京博爱医院内科 北京市 100068
  • 收稿日期:2004-03-29 出版日期:2004-09-25 发布日期:2004-09-25

Effect of Shuanglong pill on expression of vascular endothelial growth factor(VEGF) and basic fibroblast growth factor(bFGF) on aortic atherosclerotic plaque

XUE Jin-gui, HU Wang-ying, YANG Zhu-fu   

  1. Cardiology Department, Shanghai Shuguang Hospital,Shanghai 200021, China
  • Received:2004-03-29 Published:2004-09-25 Online:2004-09-25

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摘要： 目的观察双龙丸对兔主动脉粥样硬化斑块内血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)表达的影响。方法免疫损伤加高脂喂养建立兔动脉粥样硬化(AS)模型,分为空白对照组(Ⅰ组,6只)、实验对照组(Ⅱ组,6只)、开搏通对照组(Ⅲ组,6只,10mg/kg·d,灌胃12周)和双龙丸组(Ⅳ组,6只,2.52g/kg·d,灌胃12周)。免疫组织化学法定性和定量观察VEGF和bFGF在主动脉粥样斑块内表达。结果Ⅲ组和Ⅳ组VEGF和bFGF表达面积、密度和密度指数比Ⅱ组显著降低(P<0.01);Ⅳ组VEGF表达面积、密度和密度指数比Ⅲ组显著降低(P<0.01)。病理染色示,Ⅰ组内中膜未见泡沫细胞形成,无bFGF和VEGF黄染。Ⅱ组,粥样斑块内大量泡沫细胞形成,广泛淡黄染。Ⅲ组和Ⅳ组,内膜少量泡沫细胞形成,呈轻微淡黄染。结论双龙丸和开搏通一样,可通过抑制VEGF和bFGF在动脉粥样斑块内表达,延缓或逆转动脉粥样硬化进展。

关键词: 双龙丸, 动脉粥样硬化, 血管内皮生长因子(VEGF), 碱性成纤维细胞生长因子(bFGF)

Abstract: ObjectiveTo study the effect of Shuanglong pill on expression of vascular endothelial growth factor(VEGF) and basic fibroblast growth factor(bFGF) on aortic atherosclerotic plaque.MethodsAs atherosclerotic(AS) models of rabbits were made by immune injury plus hyperlipid diet, all rabbits were divided into blank control group(group Ⅰ,6), experimental control group(group Ⅱ, 6), captopril control group(group Ⅲ, 6; 10 mg/kg·d,12 weeks), and Shuanglong pill group(group Ⅳ, 6; 2.52 g/kg·d,12 weeks). Quantitive and qualitative analysis of expression of VEGF and BFGF on aortic atherosclerotic plaque were performed.ResultsExpressive area, density and density index of VEGF and bFGF on aortic atherosclerotic plaques were significantly decreased in group Ⅲ and Ⅳ than in group Ⅱ(P<0.01). There were significant decrease for VEGF expression in group Ⅳ than in group Ⅲ(P<0.01).Pathologic staining showed that there were no foam cells formation and no stained yellow in group Ⅰ, but mass foam cells formation and widespread stained yellow in group Ⅱ. There were a small quantity of foam cells formation and little stained yellow in group Ⅲ and group Ⅳ.ConclusionShuanglong pill can delay/reverse AS progression by inhibiting VEGF and bFGF expression in atherosclerotic plaque as well as captopril.

Key words: Shuanglong pill, atherosclerosis, vascular endothelial growth factor (VEGF), basic fibroblast growth factor(bFGF)