《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (05): 271-273.

• 基础研究 • 上一篇    下一篇

血管平滑肌β-肾上腺能受体对细胞凋亡的影响

马晶1,2; 郑铭2; 王士雯1; 赵玉生1   

  1. 1.解放军总医院老年心血管病研究所 北京市 100853;2.北京大学医学部心血管病研究所 北京市 100038
  • 收稿日期:2004-03-15 出版日期:2004-05-25 发布日期:2004-05-25

Effect of β-adrenergic receptor stimulation on vascular smooth muscle cell apoptosis

MA Jing, ZHENG Ming, WANG Shi-wen, et al   

  1. Institute of Geriatric Cardiology, General Hospital of PLA, Beijing 100853, China
  • Received:2004-03-15 Published:2004-05-25 Online:2004-05-25

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摘要： 目的探讨刺激生理状态及过表达的血管平滑肌细胞β2肾上腺能受体(VSMCβ2AR)对细胞凋亡的影响。方法应用腺病毒介导的转基因技术过表达VSMCβ2AR,采用Hoechst 33342染色和流式细胞仪分析生理状态下和β2AR过表达活化后细胞凋亡情况。结果应用异丙基肾上腺素刺激VSMCβ2 AR24h后,流式细胞仪检测存活VSMC数量与对照组相比较并无明显减少,48h后较对照组明显减少(P<0.01);过表达β2AR的VSMC应用异丙基肾上腺素刺激24h后,流式细胞仪检测细胞存活率明显下降(P<0.01),Hoechst染色显示凋亡细胞率明显升高(P<0.01)。结论刺激生理状态及过表达的VSMCβ2 AR具有促进凋亡的效应。

关键词: β-肾上腺素能受体, 血管平滑肌细胞, 凋亡

Abstract: ObjectiveTo investigate the effect of β-adrenergic receptor(β2AR) stimulation on vascular smooth muscle cell apoptosis in physiological state and receptor overexpression model.Methodsβ2AR overexpression model was established by transgenic techniques. Hoechst 33342 staining as well as flow cytometer(FCM) detected were chosen to measure the incidence of vascular smooth muscle cell apoptosis.ResultsVascular smooth muscle cell exhibited significantly fewer viable cell rate when stimulated with β2AR agonist isoproteronol for 48 hours compared with control (P<0.01),while no difference at the time point of 24 hours. Much fewer viable rate detected by FCM and high apoptotic rate by Hoechst staining were observed when VSMCs overexpressing β2AR were stimulated with isoproteronol for 24 hours (P<0.01).ConclusionStimulation of physiological and overexpressing β2AR could induce apoptosis of vascular smooth muscle cell.

Key words: β-adrenergic receptor, vascular smooth muscle cell, apoptosis