《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (07): 387-388.

• 专题 • 上一篇    下一篇

Schwann细胞移植对电针损伤大鼠中脑新生髓鞘的影响

万虹; 翟晶; 历俊华; 张亚卓; 王忠诚   

  1. 北京市神经外科研究所 北京市 100050
  • 收稿日期:2004-05-17 出版日期:2004-07-25 发布日期:2004-07-25

Effect of Schwann cells transplanting into injured middle brain on remyelination in rat

WAN Hong, ZHAI Jing, LI Jun-hua, et al   

  1. Beijing Neurosurgical Institute, Beijing 100050, China
  • Received:2004-05-17 Published:2004-07-25 Online:2004-07-25

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摘要： 目的观察SchWann细胞移植于电针损伤的中脑后是否有新生的髓鞘。方法新生大鼠坐骨神经SchWann细胞体外培养,5溴-脱氧尿嘧啶(BrdU)标记后移植至电针损伤的大鼠中脑网状结构。免疫组织化学染色定位后行半薄切片,天青-美蓝髓鞘染色,油镜下观察新生的髓鞘。结果SchWann细胞移植后8个月仍可见BrdU阳性细胞,并主要向大脑皮层迁移;在损伤的脑干区域内,移植后1个月就可见新生的髓鞘。结论SchWann细胞移植可促进损伤的中枢神经系统再生。

关键词: Schwann细胞, 中脑, 髓鞘再生, 细胞移植

Abstract: Objective To investigate the remyelination after Schwann cells grafted into injured middle brain in rat.Methods Schwann cells originated from sciatic nerves of 1 to 2 day old rat were expanded and labeled by BrdU in vitro, transplanted into the rat middle brain injured by electric needle stimulus. Immunohistochemistry and myelin staining were used to locate in the BrdU positive cells and investigate remyelination.Results BrdU positive cells could be identified till at least 8 months after grafting, which mainly migrated toward injured ipsilateral cortex. New myelination could be seen in destructed brain stem area. Conclusion Schwann cells transplantation could promote CNS regeneration.

Key words: Schwann cell, middle brain, remyelination, cells transplantation