《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (04): 245-246.

• 经验交流 • 上一篇    下一篇

血管内直接线栓闭塞法制备大鼠缺血性脑梗死模型

宋红松1; 樊东升1; 王荫华2   

  1. 1.北京大学第三医院神经内科 北京市 100083;2.北京大学第一医院神经内科 北京市 100034
  • 收稿日期:2004-02-12 出版日期:2004-04-25 发布日期:2004-04-25

Rat cerebral ischemia infarct model made by thread blocked directly in blood vessel

SONG Hong-song, FAN Dong-sheng, WANG Yin-hua   

  1. Department of Neurology, the Third Hospital, Peking University, Beijing 100083, China
  • Received:2004-02-12 Published:2004-04-25 Online:2004-04-25

摘要: 目的规范血管内直接线栓闭塞法制作大鼠缺血性脑梗死模型的流程,节约时间和材料。方法采用Koizumi的方法制作动物模型,重点分析关键步骤的注意事项。结果采用该方法制作动物模型时注意线拴制作、动物麻醉、手术操作细节等,手术时间容易控制,大鼠脑梗死体积明确。结论采用Koizumi的方法可以获得稳定而可靠的模型,便于条件有限的初学者掌握。

关键词: 大鼠, 脑缺血, 动物模型

Abstract: ObjectiveTo standardize the process in making the model of acute regional cerebral ischemia in Sprague-Dawley rats, and to economize time and material.MethodsRegional cerebral ischemia rat's models were induced and modified according to Koizumi's method.ResultsThe time duration was controllable and the volume of cerebral infarct was determined by adverting the approaches such as the preparation of suture, anaesthesia of the animal, and the details of surgical operation.ConclusionThe acute regional cerebral ischemic model in rats made by Koizumi's method is stable and reliable, and is easy for the beginner to carry out under limited conditions.

Key words: rat, cerebral ischemia, animal model