《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (02): 141-142.

• 基础研究 • 上一篇    下一篇

三氧化二砷诱导类风湿关节炎滑膜细胞凋亡作用的研究

吕昊哲1;乔石钰2;陈秀丽2   

  1. 1. 大庆油田总医院,黑龙江大庆市 163001;2. 大庆油田总医院集团乘风医院,黑龙江大庆市 163411
  • 收稿日期:2008-08-18 出版日期:2009-02-01 发布日期:2009-02-01

Study on the Apoptosis of Rheumatoid Arthritis Synoviocyte Induced by Arsenic Trioxide

Lü Hao-zhe, QIAO Shi-yu, CHEN Xiu-li   

  1. The General Hospital of Daqing Oil Field, Daqing 163001, Heilongjiang, China
  • Received:2008-08-18 Published:2009-02-01 Online:2009-02-01

摘要: 目的 观察三氧化二砷(ATO)对人类风湿关节炎成纤维样滑膜细胞(HFLS-RA)凋亡的作用。方法 将HFLS-RA分为单纯培养基空白对照组和0.5、2、8 μmol/L ATO实验组。ATO作用72 h后,电镜下观察细胞的病理改变,采用四唑氮法(MTT)检测ATO对HFLS-RA生长的影响。结果 ATO可以诱导HFLS-RA的凋亡,ATO呈现时间和剂量依赖性抑制HFLS-RA生长的作用。结论 ATO可以抑制HFLS-RA的生长,促进HFLS-RA的凋亡。

关键词: 三氧化二砷, 类风湿关节炎, 成纤维样滑膜细胞, 凋亡

Abstract: Objective To study the apoptosis of rheumatoid arthritis synoviocyte induced by arsenic trioxide.Methods Human rheumatoid arthritis fibroblast-like synoviocytes (HFLS-RA) were divided into control group and arsenic trioxide group. After 72 hours, pathology changes were observed and MTT tests were used to investigate apoptosis levels induced by arsenic trioxide.Results Arsenic trioxide can induce the apoptosis of rheumatoid arthritis synoviocyte, depending on time and dose.Conclusion Arsenic trioxide may induce apoptosis of rheumatoid arthritis synoviocyte.

Key words: arsenic trioxide, rheumatoid arthritis, fibroblast-like synoviocyte, apoptosis