《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (02): 120-122.

• 基础研究 • 上一篇    下一篇

变性高效液相色谱检测少突胶质细胞瘤1p杂合性缺失

李守巍1,2;袁芳1;江涛1,2;王忠诚1   

  1. 1. 北京市神经外科研究所,北京市 100050;2. 首都医科大学附属北京天坛医院胶质瘤治疗中心,北京市 100050
  • 收稿日期:2008-06-23 出版日期:2009-02-01 发布日期:2009-02-01
  • 通讯作者: 江涛

Identifying 1p LOH in Oligodendroglioma with Denaturing High Performance Liquid Chromatography

LI Shou-wei, YUAN Fang, JIANG Tao, et al   

  1. Beijing Neurosurgical Institute, Beijing 100050, China
  • Received:2008-06-23 Published:2009-02-01 Online:2009-02-01

摘要: 目的 建立临床检测少突胶质细胞瘤1p杂合性缺失的手段。方法 提取肿瘤组织及患者外周血DNA,利用聚合酶链式反应扩增微卫星位点后行变性高效液相色谱检测。结果 以患者外周血作对照,可明确显示肿瘤标本微卫星位点杂合性缺失情况,从而判断患者1p杂合性缺失情况。结论 该方法能直观便捷分析出患者1p杂合性缺失情况。

关键词: 变性高效液相色谱, 少突胶质细胞瘤, 1p杂合性缺失, 预后

Abstract: Objective To set up the clinical examination method for 1p loss of heterozygosity (LOH).Methods Genomic DNA was isolated from tumor tissue and peripheral blood in oligodendroglioma patients. Denaturing high performance liquid chromatography (DHPLC) was used to examine the polymerase chain reaction (PCR) Results . 1p LOH was determined by the microsatellite sites.Results 1p LOH was identified by DHPLC Results .Conclusion DHPLC can clearly identify the 1p LOH with short time in oligodendroglioma patients.

Key words: denaturing high performance liquid chromatography, oligodendroglioma, 1p loss of heterozygosity, prognosis