《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (02): 109-111.

• 专题 • 上一篇    下一篇

抑肽酶预处理对兔脊髓缺血再灌注损伤后NO及NOS的影响

程斌;王凯飞;李锋涛   

  1. 西安交通大学医学院第二附属医院骨科，陕西西安市 710004
  • 收稿日期:2008-09-01 出版日期:2009-02-01 发布日期:2009-02-01

Effect of Pretreatment of Aprotinin on Nitric Oxide and Nitric Oxide Synthase Contents after Spinal Cord Ischemia-reperfusion Injury in Rabbits

CHENG Bin, WANG Kai-fei, LI Feng-tao   

  1. The Department of Orthopaedics, 2nd Affiliated Hospital, Xi'an Jiaotong Unibversity College of Medicine, Xi'an 710004, Shaanxi, China
  • Received:2008-09-01 Published:2009-02-01 Online:2009-02-01

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被引次数

3

摘要： 目的 观察抑肽酶预处理对家兔脊髓缺血再灌注损伤后脊髓中一氧化氮（NO）及一氧化氮合酶（NOS）的影响。方法 45只新西兰大白兔随机分为抑肽酶预处理组、生理盐水组及假手术空白组，每组15只。以体外松解法建立家兔脊髓缺血模型，缺血60 min后恢复血流再灌注24 h。抑肽酶预处理组于缺血前10 min静脉注射抑肽酶3×107 IU/kg，继而用微量泵持续静脉注入抑肽酶1×107 IU/kg/h至实验结束；生理盐水组缺血再灌注时间同实验组，并用等量生理盐水代替抑肽酶；假手术空白组只暴露腹主动脉，不夹闭、不给药。分别于缺血前、缺血再灌注后8 h、24 h处死动物，取腰段脊髓做NO及NOS测定。结果 抑肽酶预处理组与生理盐水组再灌注8 h和24 h的NO、总NOS(TNOS)、诱导型NOS(iNOS)较缺血前升高（P＜0.05）；再灌注8 h时，抑肽酶预处理组与生理盐水组之间的TNOS、iNOS有显著性差异（P＜0.05），NO有非常显著性差异（P＜0.01）；再灌注24 h时，抑肽酶预处理组与生理盐水组之间的NO、TNOS、iNOS均有非常显著性差异（P＜0.01）；抑肽酶预处理组与生理盐水组在再灌注8 h、24 h时，NO、TNOS、iNOS较假手术空白组明显升高（P＜0.01）。结论 缺血再灌注时，脊髓中产生过量NO是引起脊髓损伤的重要因素，抑肽酶预处理可以降低脊髓缺血再灌注时脊髓中NO的含量，减轻再灌注损伤，对脊髓缺血再灌注损伤具有保护作用。

关键词: 抑肽酶, 脊髓, 缺血再灌注, 一氧化氮, 一氧化氮合酶

Abstract: Objective To observe the effect of pretreatment of aprotinin on nitric oxide (NO) and nitric oxide synthase (NOS) contents after ischemia-reperfusion injury of spinal cord in rabbits.Methods 45 rabbits were randomly divided into aprotinin treatment group (group A), normal saline control group (group B) and pseudo-surgical operation group (group C) with 15 rabbits in each group. The infrarenal segment in abdominal aorta was clamped for 60 min to construct the model of lumbosacral spinal cord ischemia in rabbits. Reperfusion was followed and kept on for 24 h until the blood flow regained normal. Aprotinin was given 3×107 IU/kg as a short time intravenous injection for 10 min before ischemia, and then was drilled with micro pump by 1×107 IU/kg/h. Normal saline was used in group B, the ischemia-reperfusion duration between group A and group B remained same. The group C was only exposured abdominal aorta and not clamped. The rabbits were killed before ischemia and at 8 h, 24 h after ischemia-reperfusion, lumbar segment spinal cords were harvested to detect contents of NO and NOS of spinal cord.Results After 8 h of ischemia-reperfusion,the contents of NO, total NOS (TNOS), and induced NOS (iNOS) in group A and group B were more than that before ischemia (P＜0.05). After 8 h of ischemia-reperfusion, there was a significant difference in the contents of NO, TNOS, iNOS between group A and group B (P＜0.05～0.01). After 24 h of ischemia-reperfusion, there was a significant difference too between group A and group B (P＜0.01). After 8 h and 24 h ischemia-reperfusion, the contents of NO, TNOS, iNOS in group A and group B were more than that in group C (P＜0.01).Conclusion During the ischemia-reperfusion, more NO produced is an important factor of spinal cord injury. Aprotinin can decrease the contents of NO and ischemia-reperfusion injury to spinal cord of rabbits.

Key words: aprotinin, spinal cord, ischemia-reperfusion, nitric oxide, nitric oxide synthase