《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (08): 475-476.

• 基础研究 • 上一篇    下一篇

α-氨基丁酸为内标物高效液相法测定脑微透析液中氨基酸含量

张绍东1; 翟晶1; 张淑珍2; 罗芳2   

  1. 1.北京市神经外科研所 北京市 100050;2.首都医科大学附属天坛医院麻醉科 北京市 100050
  • 收稿日期:2004-02-24 出版日期:2004-08-25 发布日期:2004-08-25

Amino acids in brain microdialysate tested by HPLC with α-aminobutyric acid as the internal standard

ZHANG Shao-dong, ZHAI Jing, ZHANG Shu-zhen, et al   

  1. Department of Biochemistry, Beijing Neurosurgical Institute, Beijing 100050, China
  • Received:2004-02-24 Published:2004-08-25 Online:2004-08-25

PDF (PC)

被引次数

19

摘要： 目的研究以α-氨基丁酸(AABA)为内标物,用高效液相色谱仪测定大鼠脑微透析液中的神经递质类氨基酸含量。方法以AABA为内标,用高效液相色谱仪测定大鼠脑微透析液中的神经递质类氨基酸含量,采用OPA柱前衍生,ODS-C18色谱柱,荧光检测器,AABA作为内标,使用磷酸缓冲液和甲醇二元梯度洗脱方法,35min内完成。结果氨基酸与内标在给定条件下分离良好,每种氨基酸的回收率为88.2%—102.3%,线性回归相关系数平均为0.998±0.0015,每种氨基酸的检测限为1.0—8.6PPm。结论本方法准确、灵敏,可用于微透析样品中氨基酸的测定。

关键词: 氨基酸, 高效液相(HPLC), 微透析, α-氨基丁酸(AABA)

Abstract: ObjectiveTo study the effect of testing amino acids levels in brain microdialysate by method of high performance liquid chromatography (HPLC) with α-aminobutyric acid (AABA) as the internal standard.MethodsReversed phase high performance liquid chromatography (RP-HPLC), orthophthaladehyde (OPA) precolumn derivatization method and fluorescence detector were used. The separation was performed using an ODS-C18 column. AABA, an isomeric compound of GABA, served as the internal standard, phosphate buffer solution and methanol as the mobile phase, gradient elution lasted in 35 min.Results7 kinds of amino acids and internal standard were separated completely. The mean recovery of amino acid was 88.2%—102.3%. The mean correlation coeffeicient of the linear relationship was 0.998±0.0015.Limits of detection for amino acids were 1.0—8.6 ppm respectively.ConclusionThe method mentioned above is simple and sensitive for operation, and can be used for determined levels of amino acids in microdialysate.

Key words: amino acids, high performance liquid chromatography (HPLC), microdialysis, α-aminobutyric acid (AABA)