《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (05): 434-435.

• 基础研究 • 上一篇    下一篇

促红细胞生成素对兔脊髓缺血再灌注损伤后核因子-κB表达的影响

杜巍;梁丽珍;周巧玲;黄秋霞   

  1. 东莞市人民医院心胸外科， 广东东莞市， 523018
  • 收稿日期:2009-04-02 出版日期:2009-05-01 发布日期:2009-05-01

Effect of Erythropoietin on Expression of Nuclear Factor-κB in Rabbit Spinal Cord Tissue after Ischemia-Reperfusion

DU Wei, LIANG Li-zhen, ZHOU Qiao-ling, et al   

  1. Department of Thoracic and Cardiovascular Surgery， Dongguan People's Hospital, Dongguan 523018, Guangdong, China
  • Received:2009-04-02 Published:2009-05-01 Online:2009-05-01

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摘要： 目的 探讨促红细胞生成素（EPO）对兔脊髓缺血再灌注损伤后神经功能及核因子-κB(NF-κB)表达的影响。方法 建立兔脊髓缺血再灌注损伤模型，应用Tarlov评分法、免疫组化方法对各组动物后肢神经功能及脊髓组织NF-κB表达变化进行观察。结果 促红细胞生成素治疗组神经功能恢复较缺血再灌注组明显提高(P＜0.01)，NF-κB表达明显低于缺血再灌注组(P＜0.01)。结论 EPO对缺血再灌注损伤的脊髓具有保护作用，其作用机制与抑制NF-κB表达有关。

关键词: 脊髓, 缺血再灌注, 促红细胞生成素, 核因子-κB

Abstract: Objective To explore the effects of erythropoietin (EPO) on the neural function recovery and the expressions of nuclear factor-κB (NF-κB) in rabbit spinal cord after ischemia-reperfusion injury. Methods 24 rabbits were randomized into 3 groups: EPO treatment group, ischemia-reperfusion (I/R) group and sham group with 8 rabbits in each group. The rabbit model of spinal cord ischemia-reperfusion injury was established with Tetik's method. The changes of neural functional recovery of rabbits of 3 groups were observed through Tarlov's scale at different time points post-operation. The expressions of NF-κB were tested with immunohistochemistry. Results The grade of nerve function improved distinctly in EPO treatment group (P<0.01).The expression of NF-κB in EPO group was lower than that of I/R group (P<0.01).Conclusion EPO can significantly improve the motor function of hind limbs in rabbits after spinal cord ischemia-reperfusion injury, which might be related with inhibition of the expressions of NF-κB.

Key words: spinal cord, ischemia-reperfusion, erythropoietin, nuclear factor-κB (NF-κB)