《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (04): 330-332.

• 基础研究 • 上一篇    下一篇

抑肽酶预处理对兔脊髓缺血再灌注损伤后NO、NOS及氧自由基的影响

李锋涛1a;程斌1b;贺西京1a   

  1. 1.西安交通大学医学院第二附属医院a.骨二科;b.骨科，陕西西安市 710004
  • 收稿日期:2008-11-25 出版日期:2009-04-01 发布日期:2009-04-01

Effect of Aprotinin Preconditioning on Nitric Oxide, Nitric Oxide Synthase and Oxyradical During Spinal Cord Ischemia-reperfusion Injury in Rabbits

LI Feng-tao, CHENG Bin, HE Xi-jing   

  1. The Department of the Second Orthopedics, the Second Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Xi'an 710004, Shaanxi, China
  • Received:2008-11-25 Published:2009-04-01 Online:2009-04-01

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摘要： 目的 观察抑肽酶预处理对兔脊髓缺血再灌注损伤后一氧化氮（NO）、一氧化氮合酶（NOS）及氧自由基的影响。方法 21只国产大耳白兔随机分为实验组（8只）、对照组（8只）和假手术组（5只）。实验组缺血60 min，再灌注24 h，并于缺血前10 min静脉注射抑肽酶3×107 IU/kg体重，继而用Graseby3500微量泵持续注入抑肽酶1×107 IU/kg体重/h直至实验结束。对照组缺血及再灌注时间、方法同实验组（用生理盐水代替抑肽酶）。假手术组只暴露腹主动脉，不夹闭，不给药。缺血前、缺血再灌注后8 h、24 h处死动物，取腰段脊髓（L2～L4）进行NO及诱导型一氧化氮合酶（iNOS）测定。处死动物前取动脉血进行丙二醛（MDA）及超氧化物歧化酶（SOD）检测。结果 再灌注8 h后，实验组的NO、MDA含量和iNOS活力较对照组明显下降，SOD活力增加。结论 抑肽酶预处理可以明显抑制缺血再灌注后脊髓组织中iNOS的产生，降低NO、MDA含量，提高SOD活力，减少再灌注损伤，保护神经组织。

关键词: 抑肽酶, 脊髓, 缺血再灌注, 一氧化氮, 一氧化氮合酶, 丙二醛, 超氧化物歧化酶

Abstract: Objective To observe the effect of aprotinin preconditioning on nitric oxide (NO), nitric oxide synthase (NOS) and oxyradical during spinal cord ischemia-reperfusion injury in rabbits.Methods 21 rabbits were randomly divided into the aprotinin treatment group (8 rabbits), control group (8 rabbits) and sham operative group (5 rabbits). The infrarenal segment in abdominal aorta was clamped for 60 min to construct the model of lumbosacral spinal cord ischemia in rabbits. Reperfusion was followed and kept on for 24 h until the blood flow regained normal. In the treatment group, aprotinin was given at 3×107 IU/kg as a short time intravenous injection for 10 min before ischemia, and then was drilled with micro pump by 1×107 IU/kg/h. Normal saline was used in the control group, the ischemia-reperfusion duration between aprotinin treatment group and control group remained same. The sham operative group was only exposured abdominal aorta and not clamped. The rabbits were killed before ischemia and 8 h, 24 h after ischemia-reperfusion, lumbar segment was harvested to detect content of NO, malondialdehyde (MDA), induced nitric oxide synthase (iNOS) and superoxide dismutase (SOD) of spinal cord.Results 8 h after spinal cord ischemia-reperfusion, compared with the control group, the content of NO, MDA and the activity of iNOS were less, and the activity of SOD was more in the aprotinin treatment group ( P<0.05).Conclusion Aprotinin pretreatment can reduce the content of NO, MDA and descend the activity of NOS. Moreover aprotinin pretreatment can ascend the activity of SOD and improve apoptosis of nerve cell.

Key words: aprotinin, spinal cord, ischemia-reperfusion, nitric oxid, nitric oxide synthase, malondialdehyde, superoxide dismutase